Ungar B L, Yolken R H, Quinn T C
Am J Trop Med Hyg. 1985 May;34(3):465-72. doi: 10.4269/ajtmh.1985.34.465.
An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Entamoeba histolytica in human feces, using both a monoclonal antibody and rabbit antisera. It detected from less than 1 to 57 trophozoites of 6 E. histolytica strains. Stool specimens were positive by ELISA in 18 of 22 (82%) patients with E. histolytica and in 3 of 186 (2%) of patients without demonstrable E. histolytica in their stools. The latter included one from a child living near an asymptomatic cyst carrier and another from a traveler with giardiasis who had recently taken antibiotics. One hundred eight of 183 microscopy-and ELISA-negative specimens contained other parasites including Giardia (49 specimens), Endolimax nana (24), Entamoeba coli (21), Iodamoeba butschlii (2), and Entamoeba hartmanni (1). This ELISA for E. histolytica is a simple, sensitive and specific diagnostic tool.
采用单克隆抗体和兔抗血清，开发了一种间接酶联免疫吸附测定（ELISA）法用于检测人粪便中的溶组织内阿米巴。该方法可检测6株溶组织内阿米巴菌株中少于1至57个滋养体。在22例溶组织内阿米巴患者中，有18例（82%）粪便标本经ELISA检测呈阳性；在186例粪便中未检出溶组织内阿米巴的患者中，有3例（2%）呈阳性。后者包括一名居住在无症状包囊携带者附近的儿童以及一名近期服用过抗生素的贾第虫病旅行者。183份显微镜检查和ELISA检测均为阴性的标本中，有108份含有其他寄生虫，包括贾第虫（49份标本）、微小内蜒阿米巴（24份）、结肠内阿米巴（21份）、布氏嗜碘阿米巴（2份）和哈氏内阿米巴（1份）。这种用于检测溶组织内阿米巴的ELISA法是一种简单、灵敏且特异的诊断工具。
