Zemła Agata, Grzegorek Irmina, Dzięgiel Piotr, Jabłońska Karolina
Department of Histology and Embriology, Wroclaw Medical University, Wroclaw, Poland.
Department of Histology and Embriology, Wroclaw Medical University, Wroclaw, Poland
In Vivo. 2017 Sep-Oct;31(5):801-809. doi: 10.21873/invivo.11133.
BACKGROUND/AIM: Melatonin (MLT), through the interaction with membrane melatonin receptors MT1, can improve the effectiveness of cytostatic agents, including cisplatin (CP). The aim of this study was to examine the synergistic effect of MLT and CP in three cell lines: IOSE 364, SK-OV-3 and OVCAR-3, as well as to assess the role of MT1 receptors in this mechanism.
Using the SRB assay we investigated the effect of different concentrations of CP and MLT on cell viability. Tests, using luzindole - MT1 inhibitor, allowed us to assess the potential involvement of MT1 in the mechanism of MLT action.
MLT at certain concentrations demonstrated a synergistic effect in combination with CP. The addition of luzindole did not affect the action of MLT in combination with CP.
In summary, the synergistic effect of MLT with CP seems to be independent of membrane MT1 receptors.
背景/目的：褪黑素（MLT）通过与膜褪黑素受体MT1相互作用，可提高包括顺铂（CP）在内的细胞抑制剂的有效性。本研究旨在检测MLT与CP在三种细胞系（IOSE 364、SK-OV-3和OVCAR-3）中的协同作用，并评估MT1受体在该机制中的作用。
使用SRB检测法，我们研究了不同浓度的CP和MLT对细胞活力的影响。使用MT1抑制剂鲁辛朵进行的试验，使我们能够评估MT1在MLT作用机制中的潜在参与情况。
特定浓度的MLT与CP联合显示出协同作用。添加鲁辛朵并不影响MLT与CP联合的作用。
总之，MLT与CP的协同作用似乎独立于膜MT1受体。
