Zhu Xiaojing, Zhang Hui, Zhang Xinchun, Ning Chengyun, Wang Yan
Department of Prosthodontics, Guanghua School of Stomatology & Hospital of Stomatology, Guangdong Key Laboratory of Stomatology, Sun Yat-sen University.
School of Material Science and Engineering, South China University of Technology.
Dent Mater J. 2017 Sep 26;36(5):677-685. doi: 10.4012/dmj.2016-189. Epub 2017 Sep 7.
To fabricate a sustained-release delivery system of bone morphogenetic protein (BMP-2) on titanium surface, explore the effect of BMP-2 concentration on the loading/release behavior of BMP-2 and evaluate the cell compatibility of the system in vitro, pure titanium specimens were immersed into supersaturated calcium phosphate solutions (SCP) containing 4 different concentrations of BMP-2: 0, 50, 100, 200 and 400 ng/mL. Biomimetic calcium phosphate coating was formed on titanium surface and BMP-2 was incorporated into the coating through co-deposition. The release profile of BMP-2 suggested that BMP-2 were delivered sustainably up to 20 days. CCK-8 and ALP assay showed that 200 group and 400 ng/mL BMP-2 group have significant effect on promoting MC3T3-E1 cell proliferation and differentiation. The BMP-2 incorporated into the hybrid coating released in a sustained manner and significantly promoted the proliferation and differentiation of MC3T3-E1 on the titanium surface.
为了在钛表面构建骨形态发生蛋白（BMP-2）缓释递送系统，探究BMP-2浓度对其负载/释放行为的影响，并在体外评估该系统的细胞相容性，将纯钛标本浸入含有4种不同浓度BMP-2（0、50、100、200和400 ng/mL）的过饱和磷酸钙溶液（SCP）中。在钛表面形成仿生磷酸钙涂层，并通过共沉积将BMP-2掺入涂层中。BMP-2的释放曲线表明，BMP-2可持续释放长达20天。CCK-8和碱性磷酸酶检测表明，200 ng/mL组和400 ng/mL BMP-2组对促进MC3T3-E1细胞增殖和分化有显著作用。掺入混合涂层中的BMP-2持续释放，并显著促进钛表面MC3T3-E1细胞的增殖和分化。
