Departament of Morphology - Anatomy, Araraquara Dental School, UNESP, Araraquara, SP, Brazil.
Department of Diagnosis and Surgery, Araraquara Dental School, UNESP, Araraquara, SP, Brazil.
J Mater Sci Mater Med. 2021 May 17;32(6):57. doi: 10.1007/s10856-021-06528-4.
The aim of this study was to evaluate the behavior of rat bone marrow stem cells seeded on a Ti-15Mo alloy surface modified by laser-beam irradiation followed by calcium phosphate deposition.
A total of four groups were evaluated: polished commercially pure titanium (cpTi): Ti-P; laser irradiation + calcium phosphate deposition on cpTi: Ti-LCP; polished Ti-15Mo alloy: Ti15Mo-P; and laser irradiation + calcium phosphate deposition on Ti-15Mo alloy: Ti15Mo-LCP. Before and after laser irradiation and calcium phosphate deposition on the surfaces, physicochemical and morphological analyses were performed: Scanning Electron Microscopy (SEM) and Energy Dispersive Spectroscopy (EDX). The wettability of the samples was evaluated by contact angle measurement. In addition, the behavior of osteoblast-like cells to these surfaces was evaluated for cell morphology, adhesion, proliferation and viability, evaluation of alkaline phosphatase formation and gene expression of osteogenesis markers.
Surfaces wet-abrade with grit paper (P) showed oriented groves, while the laser irradiation and calcium phosphate deposition (LCP) produced porosity on both cpTi and Ti15Mo alloy groups with deposits of hydroxyapatite (HA) crystals (SEM). EDX showed no contamination after surface modification in both metal samples. A complete wetting was observed for both LCP groups, whereas P surfaces exhibited high degree of hydrophobicity. There was a statistical difference in the intragroup comparison of proliferation and viability (p < 0.05). The ALP activity showed higher values in the Ti15Mo alloy at 10 days of culture. The gene expression of bone related molecules did not present significant differences at 7 and 14 days among different metals and surface treatments.
Ti15-Mo seems to be an alternative alloy to cpTi for dental implants. Surface treatment by laser irradiation followed by phosphate deposition seems to positively interact with bone cells.
Ti-15Mo alloy surface modified by laser-beam irradiation followed by calcium phosphate deposition may improve and accelerate the osseointegration process of dental implants.
本研究旨在评估激光束辐照和磷酸钙沉积后钛-15 钼合金表面种植大鼠骨髓干细胞的行为。
共评估四组：抛光商用纯钛（cpTi）：Ti-P；激光辐照+cpTi 磷酸钙沉积：Ti-LCP；抛光 Ti-15Mo 合金：Ti15Mo-P；激光辐照+Ti-15Mo 合金磷酸钙沉积：Ti15Mo-LCP。在激光辐照和表面磷酸钙沉积前后，进行理化和形态分析：扫描电子显微镜（SEM）和能谱分析（EDX）。通过接触角测量评估样品的润湿性。此外，还评估了成骨样细胞对这些表面的行为，包括细胞形态、黏附、增殖和活力、碱性磷酸酶形成的评估以及成骨标志物的基因表达。
用砂纸打磨（P）的表面显示出定向的凹槽，而激光辐照和磷酸钙沉积（LCP）在 cpTi 和 Ti15Mo 合金组均产生了多孔性，并有羟基磷灰石（HA）晶体沉积（SEM）。EDX 显示两种金属样品表面改性后均无污染。LCP 两组均表现出完全润湿，而 P 表面表现出高度疏水性。在增殖和活力的组内比较中存在统计学差异（p<0.05）。培养 10 天时 Ti15Mo 合金的碱性磷酸酶活性较高。7 天和 14 天不同金属和表面处理之间的骨相关分子的基因表达没有显著差异。
Ti15-Mo 似乎是牙科植入物的替代合金。激光辐照后磷酸盐沉积的表面处理似乎与骨细胞有积极的相互作用。
激光束辐照和磷酸钙沉积后 Ti-15Mo 合金表面改性可能改善和加速牙科植入物的骨整合过程。
