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通过简单序列重复间标记和核糖体RNA-ITS基因测序对[物种1]和[物种2]之间体细胞杂种的分子特征分析

Molecular characterizations of somatic hybrids developed between and through inter-simple sequence repeat markers and sequencing of ribosomal RNA-ITS gene.

作者信息

Mallick Pijush, Chattaraj Shruti, Sikdar Samir Ranjan

机构信息

Division of Plant Biology, Bose Institute, P-1/12, C.I.T., Scheme VII M, Kolkata, 700 054 India.

Present Address: Unidad Académica Sistemas Arrecifales, Instituto de Ciencias del Mar y Limnología, UNAM, Prol. Avenida Niños Heroes S/N, 77580 Puerto Morelos, Quintana Roo Mexico.

出版信息

3 Biotech. 2017 Oct;7(5):298. doi: 10.1007/s13205-017-0931-2. Epub 2017 Sep 6.

DOI:10.1007/s13205-017-0931-2
PMID:28884065
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5587416/
Abstract

The 12 somatic hybrids and 2 parents of and s were characterized by ISSR and sequencing of rRNA-ITS genes. Five ISSR primers were used and amplified a total of 54 reproducible fragments with 98.14% polymorphism among all the hybrid populations and parental strains. UPGMA-based cluster exhibited a dendrogram with three major groups between the parents and hybrids. Parent . and . showed different degrees of genetic distance with all the hybrid lines and they showed closeness to hybrid and , respectively. ITS1(F) and ITS4(R) amplified the rRNA-ITS gene with 611-867 bp sequence length. The nucleotide polymorphisms were found in the ITS1, ITS2 and 5.8S rRNA region with different number of bases. Based on rRNA-ITS sequence, UPGMA cluster exhibited three distinct groups between and , and , and and .

摘要

利用ISSR和rRNA-ITS基因测序对12个体细胞杂种以及亲本 和 进行了表征。使用了5条ISSR引物，在所有杂种群体和亲本菌株中总共扩增出54个可重复片段，多态性为98.14%。基于UPGMA的聚类分析显示出一个聚类图，亲本和杂种之间分为三大类。亲本 和 与所有杂种系表现出不同程度的遗传距离，它们分别与杂种 和 亲缘关系较近。ITS1(F)和ITS4(R)扩增出rRNA-ITS基因，序列长度为611 - 867 bp。在ITS1、ITS2和5.8S rRNA区域发现了具有不同碱基数目的核苷酸多态性。基于rRNA-ITS序列，UPGMA聚类分析显示在 和 、 和 以及 和 之间分为三个不同的类群。