Razavi Bibi Marjan, Zargarani Naser, Hosseinzadeh Hossein
Targeted Drug Delivery Research Center, Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Avicenna J Phytomed. 2017 Jul-Aug;7(4):353-365.
The extract of and its main component, verbascoside, are known for their hypnotic effects in traditional medicine. In this study, the anxiolytic and hypnotic effects of leave extracts and verbascoside were evaluated in mice.
Animals were divided into 11 groups of six mice each. Group I received normal saline, Group II received Diazepam (2 mg/kg) as positive control, Groups III, IV and V received ethanolic extracts (50, 100 and 200 mg/kg, respectively), Groups VI, VII and VIII received aqueous extracts (50, 100 and 200 mg/kg, respectively) and Groups IX, X and XI received Verbascoside (25, 50 and 100 mg/kg, respectively). All agents were administrated intraperitoneally. To evaluate hypnotic activity, pentobarbital sleeping test, and for anxiolytic activity, elevated plus-maze (EPM), locomotor activity, open field and motor coordination (rotarod test) tests were used. To understand the role of GABA receptor, flumazenil was also administered.
The extracts and verbascoside increased the time spent and number of entries in the open arms of EPM. Moreover, these agents significantly increased the sleeping time induced by pentobarbital. In addition, the highest dose of extracts and verbascoside reduced time spent on the rod and total locomotion in the open field tests, respectively. Flumazenil inhibited the effects of extracts and verbascoside in EPM and hypnotic tests.
These results suggested that ethanolic and aqueous extracts of and verbascoside exhibit anxiolytic, hypnotic and muscle relaxant effects especially at the highest doses and these effects are partially due to the interaction with GABA receptor.
在传统医学中，[植物名称]提取物及其主要成分毛蕊花糖苷以其催眠作用而闻名。在本研究中，评估了[植物名称]叶提取物和毛蕊花糖苷对小鼠的抗焦虑和催眠作用。
将动物分为11组，每组6只小鼠。第一组给予生理盐水，第二组给予地西泮（2mg/kg）作为阳性对照，第三、四、五组分别给予[植物名称]乙醇提取物（50、100和200mg/kg），第六、七、八组分别给予[植物名称]水提取物（50、100和200mg/kg），第九、十、十一组分别给予毛蕊花糖苷（25、50和100mg/kg）。所有药物均腹腔注射给药。为评估催眠活性，采用戊巴比妥睡眠试验；为评估抗焦虑活性，采用高架十字迷宫（EPM）、自发活动、旷场试验和运动协调性（转棒试验）。为了解GABA受体的作用，还给予了氟马西尼。
提取物和毛蕊花糖苷增加了EPM开放臂的停留时间和进入次数。此外，这些药物显著增加了戊巴比妥诱导的睡眠时间。此外，提取物和毛蕊花糖苷的最高剂量分别减少了转棒试验中的停留时间和旷场试验中的总活动量。氟马西尼在EPM和催眠试验中抑制了提取物和毛蕊花糖苷的作用。
这些结果表明，[植物名称]的乙醇提取物、水提取物和毛蕊花糖苷具有抗焦虑、催眠和肌肉松弛作用，尤其是在最高剂量时，这些作用部分归因于与GABA受体的相互作用。
