Bonyani Atousa, Sajjadi Seyed Ebrahim, Rabbani Mohammad
Department of Pharmacology, School of Pharmacy and Pharmaceutical Science, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Science, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
Res Pharm Sci. 2018 Jun;13(3):205-212. doi: 10.4103/1735-5362.228941.
is commonly used in Iranian folk medicine for treatment of many disorders. Since there scientific data to prove the anxiolytic properties of this plant in Iran are scarce, we aimed to evaluate the sedative and anxiolytic activity of the leaf extract and essence of in an animal model of anxiety. The extract and the essence used were obtained after maceration and hydro-distillation of the leaves of , respectively. We evaluated the anti-anxiety profile and sedative activity of diazepam (1 mg/kg i.p. as the standard), hydroethanolic extract (200 and 400 mg/kg i.p.) and the essence (10, 15, and 50 mg/kg i.p.) of leaves of using elevated plus-maze and locomotor activity. We also used flumazenil, to find out if the possible effects are mediated through gamma-aminobutyric acid (GABA)/benzodiazepine receptor complex. The results showed that the essence of at a dose of 15 mg/kg is the most effective anxiolytic dose. Interestingly, flumazenil reversed this action of the essence as well as that of diazepam. The extract even at a dose of 400 mg/kg did not show significant anxiolytic effect. In locomotor activity studies, the essence caused sedation to a lesser extent than diazepam. The results suggest that the essence of this plant could be a better candidate for further analysis and fractionation. As the anxiolytic effect of the essence is reversed by flumazenil, it is possible that the GABA receptor could be involved in mediating these effects.
在伊朗民间医学中，它常用于治疗多种疾病。由于在伊朗缺乏科学数据来证明这种植物的抗焦虑特性，我们旨在评估该植物叶提取物和精油在焦虑动物模型中的镇静和抗焦虑活性。所用提取物和精油分别是通过对该植物叶子进行浸渍和水蒸馏获得的。我们使用高架十字迷宫和自发活动实验，评估了地西泮（腹腔注射1mg/kg作为标准）、乙醇提取物（腹腔注射200和400mg/kg）以及该植物叶精油（腹腔注射10、15和50mg/kg）的抗焦虑作用和镇静活性。我们还使用氟马西尼来探究这些可能的作用是否通过γ-氨基丁酸（GABA）/苯二氮䓬受体复合物介导。结果表明，15mg/kg剂量的该植物精油是最有效的抗焦虑剂量。有趣的是，氟马西尼可逆转该精油以及地西泮的这种作用。即使是400mg/kg剂量的提取物也未显示出显著的抗焦虑作用。在自发活动研究中，该精油引起的镇静作用程度低于地西泮。结果表明，这种植物的精油可能是进一步分析和分离的更好选择。由于该精油的抗焦虑作用可被氟马西尼逆转，GABA受体可能参与介导这些作用。
