The effects of lethal zinc concentrations on some physiological and biochemical parameters in Clarias lazera and Tilapia zilli were investigated. 2. The analyses of lactate, pyruvate and glycogen in both liver and muscle tissues and the relation among them have been studied in detail. 3. Significant increases were observed in liver and serum proteins, serum alkaline phosphatase (ALP), erythrocyte count (RBCs), haematocrit or packed cell volume (PCV) and haemoglobin (HB) concentrations. 4. Zinc exposure reduced liver and serum acid phosphatase (ACP) as well as liver alkaline phosphatase (ALP).