Gao Kailun, Wang Zhicheng, Zhou Xiaoxu, Wang Haoze, Kong Derong, Jiang Chen, Wang Xiuli, Jiang Zhiqiang, Qiu Xuemei
College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2017 Dec;24:79-88. doi: 10.1016/j.cbd.2017.08.002. Epub 2017 Aug 30.
Fast twitch muscle and slow twitch muscle are two important organs of Takifugu rubripes. Both tissues are of ectodermic origin, and the differences between the two muscle fibers reflect the differences in their myofibril protein composition and molecular structure. In order to identify and characterize the gene expression profile in the two muscle fibers of T. rubripes, we generated 54 million and 44 million clean reads from the fast twitch muscle and slow twitch muscle, respectively, using RNA-Seq and identified a total of 580 fast-muscle-specific genes, 1533 slow-muscle-specific genes and 11,806 genes expressed by both muscles. Comparative transcriptome analysis of fast and slow twitch muscles allowed the identification of 1508 differentially expressed genes, of which 34 myosin and 30 ubiquitin family genes were determined. These differentially expressed genes (DEGs) were also analyzed by Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. In addition, alternative splicing analysis was also performed. The generation of larger-scale transcriptomic data presented in this work would enrich the genetic resources of Takifugu rubripes, which could be valuable to comparative studies of muscles.
快肌和慢肌是红鳍东方鲀的两个重要器官。这两种组织均起源于外胚层，两种肌纤维之间的差异反映了它们肌原纤维蛋白质组成和分子结构的差异。为了鉴定和表征红鳍东方鲀两种肌纤维中的基因表达谱，我们分别使用RNA测序从快肌和慢肌中产生了5400万和4400万条clean reads，并总共鉴定出580个快肌特异性基因、1533个慢肌特异性基因以及11806个在两种肌肉中均表达的基因。对快肌和慢肌进行比较转录组分析，鉴定出1508个差异表达基因，其中确定了34个肌球蛋白基因和30个泛素家族基因。还通过本体论（GO）分析和京都基因与基因组百科全书（KEGG）途径对这些差异表达基因（DEG）进行了分析。此外，还进行了可变剪接分析。这项工作中呈现的大规模转录组数据的产生将丰富红鳍东方鲀的遗传资源，这对于肌肉的比较研究可能具有重要价值。
