Wang Zhicheng, Qiu Xuemei, Kong Derong, Zhou Xiaoxu, Guo Zhongbao, Gao Changfu, Ma Shuai, Hao Weiwei, Jiang Zhiqiang, Liu Shengcong, Zhang Tao, Meng Xuesong, Wang Xiuli
College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2017 Jun;22:50-57. doi: 10.1016/j.cbd.2017.02.002. Epub 2017 Feb 4.
Takifugu rubripes is a classical model organism for studying the role of gonad organogenesis in such physiological processes as fertilization, sex determination, and sexual development. To explicitly investigate the mechanism associated with gonad organogenesis in T. rubripes, we obtained 44.3 million and 55.2 million raw reads from the testis and ovary, respectively, by RNA-seq and from these, 18,523 genes were identified. A total of 680 differentially expressed genes were obtained from comparison transcriptome analysis between the testis and ovary, and of these, 556 genes were up-regulated in the testis, whereas only 124 genes were upregulated in the ovary. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that many of these genes encode proteins involved in signal transduction and gonad development. We mainly focused on the differentially expressed genes that have the potential to develop into the gonad. The generation of large scale transcriptomic data presented in this work would enrich the genetic resources of T. rubripes, which should be valuable to the comparative and evolutionary studies of teleosts.
红鳍东方鲀是研究性腺器官发生在受精、性别决定和性发育等生理过程中作用的经典模式生物。为了明确探究红鳍东方鲀性腺器官发生的相关机制，我们通过RNA测序分别从睾丸和卵巢中获得了4430万和5520万条原始读数，并从中鉴定出18523个基因。通过睾丸和卵巢之间的比较转录组分析，共获得680个差异表达基因，其中556个基因在睾丸中上调，而在卵巢中上调的基因只有124个。基因本体论（GO）和京都基因与基因组百科全书（KEGG）通路分析表明，这些基因中的许多编码参与信号转导和性腺发育的蛋白质。我们主要关注有潜力发育成性腺的差异表达基因。这项工作中呈现的大规模转录组数据的产生将丰富红鳍东方鲀的遗传资源，这对硬骨鱼类的比较和进化研究应该是有价值的。
