豚鼠S抗原诱导的实验性自身免疫性葡萄膜炎中组织病理学、临床和生化参数之间的相关性

Mahlberg K, Uusitalo H, Palkama A, Tallberg T

Laboratory of Immunology, Helsinki University Central Hospital, Finland.

Exp Eye Res. 1987 Jul;45(1):157-67. doi: 10.1016/s0014-4835(87)80086-6.

Footpad immunization with purified bovine retinal S-antigen in complete Freund's adjuvant was used to induce experimental allergic uveitis in 32 guinea-pigs. Thirteen animals immunized only with complete Freund's adjuvant were used as controls. Ninety-three point seven per cent of the animals immunized with S antigen developed clinical experimental allergic uveitis while none of the controls had any clinical manifestation of uveitis. Histopathological analysis in the experimental group revealed mono- and polymorphonuclear cell infiltration in the choroid, ciliary body and iris. Simultaneous disruption of the outer segment of photoreceptor cell layer was also noticed. For the anterior segment of the eye there was a strong correlation between the histopathological grading and the clinical grading. For the posterior segment this correlation was, however, poor. Histopathological changes in the eye were correlated with the clinical grading and biochemical parameters (phospholipase A2, prostaglandin E2, leukotriene C4, proteins and myeloperoxidase) measured from aqueous humour, serum samples and ciliary body-iris homogenate. Protein and phospholipase A2 levels in the aqueous humour correlated well with the histological grading of the anterior segment of the eye. The myeloperoxidase activity, measured from ciliary body-iris homogenate, also correlated with the inflammatory cell infiltration in the anterior segment. Leukotriene C4 and prostaglandin E2 levels in aqueous humour did not correlate with the histopathological, or with the clinical grading, although elevated mean values were recorded in eyes having uveitis. Gamma glutamyl transpeptidase, measured in the serum, had a poor correlation with the histological grading for the anterior segment. All other parameters, measured in serum, did not correlate with the histopathological or the clinical grading. Histopathological changes in the anterior uvea are thus reflected by elevated protein and phospholipase A2 levels in aqueous humour as well as by myeloperoxidase activity in ciliary body-iris homogenate.

用纯化的牛视网膜S抗原与完全弗氏佐剂进行足垫免疫，以诱导32只豚鼠发生实验性过敏性葡萄膜炎。13只仅用完全弗氏佐剂免疫的动物作为对照。用S抗原免疫的动物中有93.7%发生了临床实验性过敏性葡萄膜炎，而对照组动物均无葡萄膜炎的任何临床表现。实验组的组织病理学分析显示脉络膜、睫状体和虹膜有单核细胞和多形核细胞浸润。同时还注意到光感受器细胞层外段的破坏。对于眼前段，组织病理学分级与临床分级之间有很强的相关性。然而，对于眼后段，这种相关性较差。眼部的组织病理学变化与临床分级以及从房水、血清样本和睫状体 - 虹膜匀浆中测得的生化参数（磷脂酶A2、前列腺素E2、白三烯C4、蛋白质和髓过氧化物酶）相关。房水中蛋白质和磷脂酶A2水平与眼前段的组织学分级密切相关。从睫状体 - 虹膜匀浆中测得的髓过氧化物酶活性也与眼前段的炎性细胞浸润相关。房水中白三烯C4和前列腺素E2水平与组织病理学分级或临床分级均无相关性，尽管在患有葡萄膜炎的眼中记录到平均值升高。血清中测得的γ - 谷氨酰转肽酶与眼前段的组织学分级相关性较差。血清中测得的所有其他参数与组织病理学分级或临床分级均无相关性。因此，眼前葡萄膜的组织病理学变化可通过房水中蛋白质和磷脂酶A2水平升高以及睫状体 - 虹膜匀浆中的髓过氧化物酶活性反映出来。