Mahlberg K, Uusitalo R, Palkama A, Tallberg T
Curr Eye Res. 1987 Feb;6(2):321-35. doi: 10.3109/02713688709025184.
Purified S-antigen was used to induce experimental autoimmune uveitis in 42 guinea pigs. 16 animals were used as controls. Footpad immunization with fresh bovine S-antigen in physiologic saline mixed with complete Freunds's adjuvant induced a clinical disease in 95% of the eyes in test animals. In aqueous humour the increase in phospholipase-A2 and proteins as well as myeloperoxidase measured from iris-ciliary blocks correlated well with the severity of uveitis evaluated by clinical grading. Leukotriene C4 and prostaglandin E2 were only elevated in aqueous humour drawn from eyes showing a mild form of uveitis. Neither leukotriene C4 nor prostaglandin E2 were detected in eyes graded as clinically moderate or severe. In serum samples phospholipase A2, leukotriene C4, prostaglandin E2 and gamma glutamyl transpeptidase were measured. Of these biochemical parameters, only gamma glutamyl transpeptidase was significantly elevated in test animals with experimental autoimmune uveitis. Histological analysis revealed focal mononuclear cell infiltrations in the choroid. Mononuclear as well as polymorphonuclear cell infiltration was seen predominantly in the pars plana region of the ciliary body of test animals with uveitis. Simultaneous destruction of the outer segments of the photoreceptor layer was seen.
用纯化的S抗原诱导42只豚鼠发生实验性自身免疫性葡萄膜炎。16只动物作为对照。用新鲜牛S抗原与生理盐水混合并加入完全弗氏佐剂进行足垫免疫，在试验动物中95%的眼睛诱发了临床疾病。在房水中，从虹膜睫状体测量的磷脂酶A2、蛋白质以及髓过氧化物酶的增加与通过临床分级评估的葡萄膜炎严重程度密切相关。白三烯C4和前列腺素E2仅在表现为轻度葡萄膜炎的眼睛的房水中升高。在临床分级为中度或重度的眼睛中未检测到白三烯C4和前列腺素E2。在血清样本中测量了磷脂酶A2、白三烯C4、前列腺素E2和γ-谷氨酰转肽酶。在这些生化参数中，只有γ-谷氨酰转肽酶在患有实验性自身免疫性葡萄膜炎的试验动物中显著升高。组织学分析显示脉络膜有局灶性单核细胞浸润。在患有葡萄膜炎的试验动物的睫状体扁平部区域主要可见单核细胞和多形核细胞浸润。同时可见光感受器层外段的破坏。
