气相色谱串联质谱法在尿甾体分析方面具有优势。
Gas chromatography tandem mass spectrometry offers advantages for urinary steroids analysis.
作者信息
Homer Natalie, Kothiya Sanjay, Rutter Alison, Walker Brian R, Andrew Ruth
机构信息
Edinburgh Clinical Research Facility Mass Spectrometry Core, Queen's Medical Research Institute, University of Edinburgh, 47, Little France Crescent, Edinburgh, EH16 2TJ, UK.
University/British Heart Foundation Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, 47, Little France Crescent, Edinburgh, EH16 2TJ, UK.
出版信息
Anal Biochem. 2017 Dec 1;538:34-37. doi: 10.1016/j.ab.2017.09.002. Epub 2017 Sep 6.
Abstract
Gas chromatography mass spectrometry has been the lynchpin of clinical assessment of steroid profiles for ∼3 decades. The improvements in assay performance offered by tandem mass spectrometry were assessed. Across the spectrum of glucocorticoid and androgen analytes tested, limits of detection and quantitation were ∼20 fold lower with triple than single quadrupole systems, but the more noticeable improvement was that signal to noise was substantially improved and the linear range wider. These benefits allowed more reliable and concomitant measurement of steroids with substantially different abundances and in smaller volumes of urine.
摘要
大约30年来，气相色谱-质谱联用一直是类固醇谱临床评估的关键技术。对串联质谱所带来的检测性能改进进行了评估。在所测试的糖皮质激素和雄激素分析物范围内，三重四极杆系统的检测限和定量限比单重四极杆系统低约20倍，但更显著的改进是信噪比大幅提高，线性范围更宽。这些优势使得能够更可靠地同时测量丰度差异很大且尿液用量更少的类固醇。
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