Bhattacharyya Purnita, Agarwal Bikash, Goswami Madhurankhi, Maiti Debasish, Baruah Sunandan, Tribedi Prosun
Department of Microbiology, Assam Don Bosco University, Tapesia, Sonapur, Assam, 782402, India.
Department of Electronics & Communication Engineering, Assam Don Bosco University, Azara, Guwahati, Assam, 781017, India.
Antonie Van Leeuwenhoek. 2018 Jan;111(1):89-99. doi: 10.1007/s10482-017-0930-7. Epub 2017 Sep 9.
Biofilms are structured consortia of microbial cells that grow on living and non living surfaces and surround themselves with secreted polymers. Infections with bacterial biofilms have emerged as a foremost public health concern because biofilm growing cells can be highly resistant to both antibiotics and host immune defenses. Zinc oxide nanoparticles have been reported as a potential antimicrobial agent, thus, in the current study, we have evaluated the antimicrobial as well as antibiofilm activity of zinc oxide nanoparticles against the bacterium Streptococcus pneumoniae which is a significant cause of disease. Zinc oxide nanoparticles showed strong antimicrobial activity against S. pneumoniae, with an MIC value of 40 μg/ml. Biofilm inhibition of S. pneumoniae was also evaluated by performing a series of experiments such as crystal violet assay, microscopic observation, protein count, EPS secretion etc. using sub-MIC concentrations (3, 6 and 12 µg/ml) of zinc oxide nanoparticles. The results showed that the sub-MIC doses of zinc oxide nanoparticles exhibited significant anti-biofilm activity against S. pneumoniae, with maximum biofilm attenuation found at 12 μg/ml. Taken together, the results indicate that zinc oxide nanoparticles can be considered as a potential agent for the inhibition of microbial biofilms.
生物膜是微生物细胞的结构化聚集体，生长在生物和非生物表面，并被分泌的聚合物所包围。细菌生物膜感染已成为一个首要的公共卫生问题，因为生物膜生长细胞对抗生素和宿主免疫防御都具有高度抗性。氧化锌纳米颗粒已被报道为一种潜在的抗菌剂，因此，在本研究中，我们评估了氧化锌纳米颗粒对肺炎链球菌（一种重要的致病细菌）的抗菌和抗生物膜活性。氧化锌纳米颗粒对肺炎链球菌显示出强大的抗菌活性，最低抑菌浓度（MIC）值为40μg/ml。还通过使用氧化锌纳米颗粒的亚最低抑菌浓度（3、6和12μg/ml）进行一系列实验，如结晶紫测定、显微镜观察、蛋白质计数、胞外聚合物分泌等，来评估肺炎链球菌的生物膜抑制情况。结果表明，氧化锌纳米颗粒的亚最低抑菌浓度剂量对肺炎链球菌表现出显著的抗生物膜活性，在12μg/ml时生物膜衰减最大。综上所述，结果表明氧化锌纳米颗粒可被视为抑制微生物生物膜的潜在药物。
