测定二鸟苷酸环化酶活性（放射性测定法）。

Determining Diguanylate Cyclase Activity (Radioactive Assay).

作者信息

Kazmierczak Barbara I

机构信息

Department of Medicine, Yale University, 333 Cedar St., New Haven, CT, 06520-8022, USA.

Department of Microbial Pathogenesis, Yale University, 333 Cedar St., New Haven, CT, 06520-8022, USA.

出版信息

Methods Mol Biol. 2017;1657:285-289. doi: 10.1007/978-1-4939-7240-1_22.

DOI:10.1007/978-1-4939-7240-1_22

PMID:28889302

Abstract

Bioinformatics approaches can identify sequence motifs associated with diguanylate cyclases (DGCs), but experimental demonstration of DGC enzymatic activity is often desired. This protocol describes a sensitive radioactive assay for DGC activity in which substrate and product are quickly and easily separated by thin-layer chromatography.

摘要

生物信息学方法可以识别与双鸟苷酸环化酶（DGCs）相关的序列基序，但通常需要对DGC酶活性进行实验证明。本方案描述了一种用于DGC活性的灵敏放射性测定法，其中底物和产物可通过薄层色谱法快速轻松地分离。