通过具有荧光检测功能的高效液相色谱技术对血浆和脑脊液中的利坦色林进行评估。

Ritanserin assessments in plasma and CSF by means of HPLC technique with fluorescence detection.

作者信息

Larsson M, Forsman A

机构信息

Psychiatric Department III, Lillhagen Hospital, University of Göteborg, Backa, Sweden.

出版信息

Methods Find Exp Clin Pharmacol. 1987 Jun;9(6):349-51.

PMID:2888936

Abstract

A specific HPLC-technique based on fluorescence detection was developed for assay of ritanserin, a serotonin receptor-blocking agent, in plasma and CSF. In 5 ml sample volumes, the limit of detection was 0.5 ng/ml, the recovery of ritanserin was 83 +/- 5%, and the inter-assay variability, expressed as the coefficient of variation, was 9.5% in the 2-788 ng/ml concentration range. When the values determined by this technique were compared with the results obtained by means of an independent HPLC-method with UV-detection, the coefficient of correlation was 0.995 (p less than 0.001). However, our technique proved to be more specific and less complicated.

摘要

开发了一种基于荧光检测的特定高效液相色谱技术，用于测定血浆和脑脊液中血清素受体阻断剂利坦色林。在5毫升样本体积中，检测限为0.5纳克/毫升，利坦色林的回收率为83±5%，在2 - 788纳克/毫升浓度范围内，批间变异（以变异系数表示）为9.5%。当将该技术测定的值与通过独立的紫外检测高效液相色谱法获得的结果进行比较时，相关系数为0.995（p小于0.001）。然而，我们的技术被证明更具特异性且更简单。