Conconi Donatella, Bentivegna Angela
School of Medicine and Surgery, University of Milano-Bicocca, Via Cadore, 48, 20900, Monza (MB), Italy.
Methods Mol Biol. 2018;1655:3-17. doi: 10.1007/978-1-4939-7234-0_1.
Here, we describe the use of complementary techniques applicable to different types of samples to analyze chromosomal alterations in urothelial carcinoma. By a conventional chromosome analysis on fresh biopsies, it is possible to delineate the status of ploidy and rough chromosomal aberrations. The multi-target fluorescence in situ hybridization (FISH) UroVysion test, for the rapid detection of chromosomal aneusomy of chromosomes 3, 7, and 17 and/or deletion of 9p21 locus, is applicable to urine specimens as well as to formalin-fixed paraffin-embedded (FFPE) specimens and fresh biopsies. Finally, array comparative genomic hybridization (array-CGH) gives the possibility of analyzing the DNA in a single experiment from a biopsy of the tumor but also from FFPE specimens; this technique is able to detect alterations at the genome level not excluding any chromosome.
在此，我们描述了适用于不同类型样本的互补技术在分析尿路上皮癌染色体改变中的应用。通过对新鲜活检组织进行常规染色体分析，可以确定倍性状态和大致的染色体畸变情况。多靶点荧光原位杂交（FISH）UroVysion检测可快速检测3号、7号和17号染色体的染色体非整倍体以及/或9p21位点的缺失，适用于尿液标本以及福尔马林固定石蜡包埋（FFPE）标本和新鲜活检组织。最后，阵列比较基因组杂交（array-CGH）能够在一次实验中分析来自肿瘤活检组织以及FFPE标本的DNA；该技术能够检测基因组水平的改变，不排除任何染色体。
