尿路上皮癌的原位小鼠模型

Orthotopic Mouse Models of Urothelial Cancer.

作者信息

Jäger Wolfgang, Moskalev Igor, Raven Peter, Goriki Akihiro, Bidnur Samir, Black Peter C

机构信息

Department of Urology and Paediatric Urology, Johannes Gutenberg-Universität Mainz, Langenbeckstraße 1, 55131, Mainz, Germany.

The Vancouver Prostate Centre, University of British Columbia, Vancouver, BC, Canada.

出版信息

Methods Mol Biol. 2018;1655:177-197. doi: 10.1007/978-1-4939-7234-0_15.

DOI:10.1007/978-1-4939-7234-0_15

PMID:28889387

Abstract

Orthotopic mouse models of urothelial cancer are essential for testing novel therapies and molecular manipulations of cell lines in vivo. These models are either established by orthotopic inoculation of human (xenograft models) or murine tumor cells (syngeneic models) in immunocompromised or immune competent mice. Current techniques rely on inoculation by intravesical instillation or direct injection into the bladder wall. Alternative models include the induction of murine bladder tumors by chemical carcinogens (BBN) or genetic engineering (GEM).

摘要

尿路上皮癌的原位小鼠模型对于在体内测试新型疗法和细胞系的分子操作至关重要。这些模型要么通过将人肿瘤细胞（异种移植模型）或鼠肿瘤细胞（同基因模型）原位接种到免疫受损或免疫健全的小鼠中建立。当前技术依赖于通过膀胱内灌注或直接注射到膀胱壁进行接种。替代模型包括通过化学致癌物（BBN）或基因工程（GEM）诱导小鼠膀胱肿瘤。