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Visualization and Quantitative Measurement of Drug-Induced Platinum Adducts in the Nuclear DNA of Individual Cells by an Immuno-Cytological Assay.

作者信息

Melnikova Margarita, Thomale Jürgen

机构信息

Institute of Cell Biology (Cancer Research), University of Duisburg-Essen Medical School, Hufelandstr. 55, 45122, Essen, Germany.

出版信息

Methods Mol Biol. 2018;1655:351-358. doi: 10.1007/978-1-4939-7234-0_24.

DOI:10.1007/978-1-4939-7234-0_24
PMID:28889396
Abstract

Immunocytological staining with adduct-specific antibodies allows the visualization and measurement of structurally defined types of DNA damage in the nuclei of individual cells. Here we describe an immunocytological assay (ICA) procedure for the localization and quantification of such damage, in particular induced by platinum-based anticancer drugs, in cell lines , in primary cell suspensions and in frozen tissue sections.

摘要

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