de Villiers E M, Wagner D, Schneider A, Wesch H, Miklaw H, Wahrendorf J, Papendick U, zur Hausen H
Referenzzentrum für humanpathogene Papillomviren, Deutsches Krebsforschungszentrum, Heidelberg.
Lancet. 1987 Sep 26;2(8561):703-6. doi: 10.1016/s0140-6736(87)91072-5.
9295 smears, obtained from women attending three gynaecological hospitals for routine screening, were examined for human papillomavirus (HPV) types 6 and 11 and HPV 16 and 18 infections by filter in-situ hybridisation. The data were compared with cytological findings. In women with normal cytological smears HPV infection was identified in about 10% of women aged between 15 and 50 years and in less than 5% of those aged over 50. In women with abnormal smears (cervical intraepithelial neoplasia [CIN] I, II, and III and invasive cancer) HPV infection was detected in 35-40%; this rate seemed to be age-independent. the Peak incidence of CIN appeared several years after that of HPV infection. In women aged greater than 30 years it also declined earlier than did HPV positivity. The age-group distribution of women with CIN I, II, and III differed significantly from that of patients with invasive cancer. Only about a third of HPV-positive patients remained virus-positive, probably because of fluctuations in virus production and the insensitivity of the test system used. It is possible that filter in-situ hybridisation underestimates the total rate of HPV infections by a factor of 2 to 3.
对来自三家妇科医院进行常规筛查的9295份涂片进行检测，通过滤膜原位杂交法检测人乳头瘤病毒（HPV）6型和11型以及HPV 16型和18型感染情况。将数据与细胞学检查结果进行比较。在细胞学涂片正常的女性中，15至50岁的女性中约10%检测出HPV感染，50岁以上的女性中感染率低于5%。在涂片异常（宫颈上皮内瘤变[CIN]I级、II级和III级以及浸润癌）的女性中，HPV感染率为35%至40%；该感染率似乎与年龄无关。CIN的发病高峰出现在HPV感染后的数年。在30岁以上的女性中，CIN的发病率也比HPV阳性率更早下降。CIN I级、II级和III级女性的年龄组分布与浸润癌患者有显著差异。只有约三分之一的HPV阳性患者仍为病毒阳性，这可能是由于病毒产生的波动以及所用检测系统的不敏感性。滤膜原位杂交法有可能将HPV感染的总发生率低估2至3倍。
