先天性梅毒新生儿全血中针对四种膜蛋白DNA的巢式聚合酶链反应（nPCR）以及针对梅毒螺旋体Tpp47 DNA的两对引物的敏感性。

Sensitivity of nPCR for four types of membrane protein DNA and of two pairs of primers for Tpp47 DNA of Treponema pallidum in whole blood of congenital syphilis newborns.

作者信息

Guan Yafei, Ding Rong, Zhou Yequn, Chen Xiaoqin, Miao Xiaolin, Zhang Ye, Luo Dan, Cui Shudong

机构信息

a Neonatal Department , The First Affiliated Hospital of Nanjing Medical University , Jiangsu , China.

b Neonatal Department , The Children Hospital of Fudan University , Shanghai , China.

出版信息

J Matern Fetal Neonatal Med. 2019 Jan;32(2):229-235. doi: 10.1080/14767058.2017.1378321. Epub 2017 Sep 22.

DOI:10.1080/14767058.2017.1378321

Abstract

BACKGROUND

Problems exist in congenital syphilis (CS) diagnosis and no single test can be used to diagnose CS.

OBJECTIVE

To know whether there exist differences of nested polymerase chain reaction (nPCR) sensitivity among four membrane protein DNA and of nPCR between two pairs of primers for Tpp47.

METHODS

We collected 30 CS patients as a study group, and 20 cases admitted to hospital for other reasons as a control group. The blood samples from newborns were left of blood for other examination, not special for the study. Following confirmed diagnosis, DNA was extracted from blood. nPCR examined for membrane protein DNA in both groups.

RESULTS

Sensitivity of nPCR for Tpp15, Tpp17, Tpp45, and Tpp47 (total) was 6/30, 3/30, 1/30, and 20/30, respectively. The sensitivity of nPCR for Tpp47 was highest among that of four types of membrane protein DNA. The nPCR sensitivity of two pairs of primers (a and b) for Tpp47 was 11/30 and 13/30, respectively. There was no significant difference between two pairs of primers for Tpp47, and total sensitivity of nPCR for Tpp47 combining Tpp47a with Tpp47b was up to 20/30.

CONCLUSION

Sensitivity of nPCR for Tpp47 was highest among that of four types of membrane protein DNA. Two pairs of primers for Tpp47 different bands could elevate nPCR sensitivity.

摘要

背景

先天性梅毒（CS）诊断存在问题，没有单一的检测方法可用于诊断CS。

目的

了解四种膜蛋白DNA的巢式聚合酶链反应（nPCR）敏感性以及Tpp47两对引物之间的nPCR是否存在差异。

方法

我们收集了30例CS患者作为研究组，20例因其他原因入院的患者作为对照组。新生儿的血样是为其他检查留存的，并非专门用于本研究。确诊后，从血液中提取DNA。两组均采用nPCR检测膜蛋白DNA。

结果

nPCR对Tpp15、Tpp17、Tpp45和Tpp47（总计）的敏感性分别为6/30、3/30、1/30和20/30。在四种膜蛋白DNA中，nPCR对Tpp47的敏感性最高。Tpp47两对引物（a和b）的nPCR敏感性分别为11/30和13/30。Tpp47的两对引物之间无显著差异，Tpp47a与Tpp47b联合的Tpp47的nPCR总敏感性高达20/30。

结论

在四种膜蛋白DNA中，nPCR对Tpp47的敏感性最高。Tpp47不同条带的两对引物可提高nPCR敏感性。