Gómez-Paz Alejandra, Drucker-Colín René, Milán-Aldaco Diana, Palomero-Rivero Marcela, Ambriz-Tututi Mónica
Departamento de Neuropatología Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico.
Hospital General Ajusco Medio "Dra. Obdulia Rodriguez Rodriguez", Unidad de, Trastornos de Movimiento y Sueño, Mexico.
Neuroscience. 2018 Sep 1;387:123-134. doi: 10.1016/j.neuroscience.2017.08.052. Epub 2017 Sep 8.
The present study evaluates the possible antinociceptive effect of chromosphere transplants in rats injected with 6-hydroxydopamine (6-OHDA), a model of Parkinson's disease. Male adult Wistar rats received 40μg/0.5μl of 6-OHDA or 0.5μl of vehicle into the left substantia nigra (SNc). Rats were evaluated for mechanical allodynia, cold allodynia, thermal hyperalgesia and formalin. Rats with altered nociceptive threshold were transplanted with chromospheres. After transplant, rats were evaluated every week. Our results confirm that 6-OHDA injection into rat's SNc reduces mechanical, thermal, and chemical thresholds. Interestingly, chromospheres' transplant reverted 6-OHDA-induced allodynia and hyperalgesia. The antinociceptive effect induced by chromospheres was dopamine D2- and opioid-receptor dependent since sulpiride or naltrexone reverted its effect.
本研究评估了在注射6-羟基多巴胺（6-OHDA）的大鼠（帕金森病模型）中进行色素球移植可能产生的抗伤害感受作用。成年雄性Wistar大鼠在左侧黑质致密部（SNc）注射40μg/0.5μl的6-OHDA或0.5μl的赋形剂。对大鼠进行机械性异常性疼痛、冷异常性疼痛、热痛觉过敏和福尔马林试验评估。伤害感受阈值改变的大鼠接受色素球移植。移植后，每周对大鼠进行评估。我们的结果证实，向大鼠SNc注射6-OHDA会降低机械、热和化学阈值。有趣的是，色素球移植逆转了6-OHDA诱导的异常性疼痛和痛觉过敏。色素球诱导的抗伤害感受作用依赖于多巴胺D2和阿片受体，因为舒必利或纳曲酮可逆转其作用。
