Research Unit, Faculty of Allied Health Sciences, The University of Lahore, Lahore, Pakistan.
University Institute of Public Health, Faculty of Allied Health Sciences, The University of Lahore, Lahore, Pakistan.
Biomed Res Int. 2021 May 5;2021:5593520. doi: 10.1155/2021/5593520. eCollection 2021.
Substance P (SP) is a peptide involved in many biological processes, including nociception and inflammation. SP has a high affinity for its receptor neurokinin-1 (NK-1R). SP/NK-1R complex plays a major role in the interactions going on during the onset of dental pain and inflammation. . To identify the expression of NK-1R in healthy and inflamed human dental pulp, as well as to identify any association with severity of dental pain. . This case-control study included ten irreversibly inflamed samples of dental pulp, which were extirpated from patients presenting with chief complaint of dental pain due to caries. Ten healthy pulps, extirpated from those teeth which were indicated for extraction due to orthodontic reasons, were used as the control group. Visual analog scale (VAS) and modified McGill Pain Questionnaire were used to assess the characteristic and severity of pain. Immunohistochemical study was performed using monoclonal antibodies against NK-1R. . The results showed that the NK-1R was expressed intensely in patients with higher pain score. The mean pain score in cases was 7.0 ± 2.0. The healthy dental pulps had negative or mild NK-1R staining of +1 intensity. The NK-1R score in cases was 2.4 ± 0.516 and 0.2 ± 0.4216 in controls. There was significant difference in NK-1R score between both groups ( value <0.05). There was a strong positive correlation between the pain score and NK-1R expression score. As the pain increased, the NK-1R expression score was also increased (0.95, value 0.000). . NK-1R is overexpressed in inflamed dental pulp. SP/NK-1R modulation may provide a novel approach for the treatment of pulpal inflammation and pain.
P 物质（SP）是一种参与多种生物学过程的肽，包括痛觉和炎症。SP 对其受体神经激肽-1（NK-1R）具有高亲和力。SP/NK-1R 复合物在牙源性疼痛和炎症发生过程中的相互作用中起主要作用。为了确定 NK-1R 在健康和发炎的人牙髓中的表达，以及确定其与牙痛严重程度的任何关联。本病例对照研究纳入了十例不可逆性牙髓炎症样本，这些样本取自因龋齿而出现牙痛主诉的患者。十例健康牙髓取自因正畸原因而拔除的牙齿，作为对照组。使用视觉模拟评分（VAS）和改良 McGill 疼痛问卷评估疼痛的特征和严重程度。使用针对 NK-1R 的单克隆抗体进行免疫组织化学研究。结果显示，NK-1R 在疼痛评分较高的患者中表达强烈。病例组的平均疼痛评分为 7.0±2.0。健康牙髓的 NK-1R 染色呈阴性或轻度+1 强度。病例组的 NK-1R 评分为 2.4±0.516，对照组为 0.2±0.4216。两组间 NK-1R 评分差异有统计学意义（ 值<0.05）。疼痛评分与 NK-1R 表达评分之间存在强正相关。随着疼痛的增加，NK-1R 表达评分也增加（0.95， 值 0.000）。NK-1R 在发炎的牙髓中过度表达。SP/NK-1R 调节可能为治疗牙髓炎症和疼痛提供一种新方法。
