College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangzhou, Guangdong, 510380, China.
Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangzhou, Guangdong, 510380, China.
Microb Pathog. 2017 Oct;111:422-430. doi: 10.1016/j.micpath.2017.09.002. Epub 2017 Sep 7.
In spite of the quite common co-infections of viruses in the cultured fish, most of the previous studies have just simply focused on the infection of a single pathogen. In this report, we observed that about 13% of cultured Chinese perch have been co-infected by infectious spleen and kidney necrosis virus (ISKNV) and Siniperca chuatsi rhabdovirus (SCRV). Furthermore, Chinese perch could co-infected by ISKNV and SCRV by intraperitoneally injection with the two viruses. Interestingly, we revealed that the two viruses could even co-infect a single cell of Chinese perch in vivo and a single Chinese perch brain cells (CPB) cell in vitro. The dynamic co-infected viruses loads in the different tissues of Chinese perch showed dependent. When CPB cells were infected with the same 10 MOI of SCRV and ISKNV, the replication of SCRV overwhelmed the replication of ISKNV. When the MOI of ISKNV (10 MOI) was 10,000 times of MOI of SCRV (0.001 MOI), the dynamic virus loads of the two viruses in CPB cells indicated that co-infections could synergistically stimulate both viruses replication at the late time points but not at early time points. The co-infections of ISKNV and SCRV in the cultured Chinese perch will shed a new light on the prevention of the viral diseases of Chinese perch. The development of multivalent vaccine which could be effective for preventing against the co-infections of the viruses is highly needed.
尽管养殖鱼类中经常存在病毒的混合感染，但之前的大多数研究都仅仅关注单一病原体的感染。在本报告中，我们观察到约 13%的养殖鳜鱼同时感染传染性脾肾坏死病毒（ISKNV）和鳜鱼虹彩病毒（SCRV）。此外，通过腹腔注射两种病毒，鳜鱼可以同时感染 ISKNV 和 SCRV。有趣的是，我们揭示了这两种病毒甚至可以在体内同时感染鳜鱼的单个细胞和单个鳜鱼脑细胞（CPB），在体外。在不同组织中，动态混合感染病毒的负荷显示出依赖性。当 CPB 细胞感染相同的 10MOI 的 SCRV 和 ISKNV 时，SCRV 的复制超过了 ISKNV 的复制。当 ISKNV 的 MOI（10MOI）是 SCRV 的 MOI（0.001MOI）的 10,000 倍时，两种病毒在 CPB 细胞中的动态病毒载量表明，混合感染可以在后期协同刺激两种病毒的复制，但在早期阶段则不会。ISKNV 和 SCRV 在养殖鳜鱼中的混合感染将为鳜鱼病毒性疾病的预防提供新的思路。开发能够有效预防两种病毒混合感染的多价疫苗是非常必要的。
