Key Laboratory of Tropical and Subtropical Fisheries Resource Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Institute, Chinese Academy of Fishery Sciences, Guangzhou 510310, China.
Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Institute, Chinese Academy of Fishery Sciences, Guangzhou 510310, China.
Cells. 2023 Dec 20;13(1):18. doi: 10.3390/cells13010018.
Mandarin fish ranavirus (MRV) infection poses a substantial challenge to the mandarin fish culture industry as no effective preventive or therapeutic measures currently exist. The creation of a highly permissive cell line from a natural host is crucial for developing a vaccine for MRV and understanding its pathogenic mechanisms. In this research, the mandarin fish () kidney cell line (SCK) was isolated from mandarin fish kidneys. Subsequently, SCK-a to SCK-g monoclonal cell lines were derived from the SCK cell population, distinguished by morphological variations. Notably, MRV infection induced an advanced cytopathic effect (CPE) in almost all cells of the SCK-f clone. Further tests showed that MRV achieved a peak viral titer of 10 50% tissue culture infectious dose (TCID)/mL and consistently exceeded 10 TCID/mL across nine passages in SCK-f cells. Electron microscopy verified the MRV virion integrity within SCK-f. In vivo experiments revealed that MRV infections led to cumulative mortality rates of 86.9% in mandarin fish and 88.9% in largemouth bass (). Such results suggest that SCK-f is highly permissive to MRV. This study underscores the importance of cellular diversity in developing viral permissive cell lines. The SCK monoclonal cell line pool may offer potential for generating highly permissive cell lines for other mandarin fish viruses.
鳜鱼弹状病毒（MRV）感染对鳜鱼养殖业构成了重大挑战，因为目前尚无有效的预防或治疗措施。从天然宿主中创建高度允许的细胞系对于开发 MRV 疫苗和了解其致病机制至关重要。在这项研究中，从鳜鱼肾脏中分离出鳜鱼（）肾细胞系（SCK）。随后，从 SCK 细胞群体中衍生出 SCK-a 至 SCK-g 单克隆细胞系，这些细胞系在形态上有所不同。值得注意的是，MRV 感染几乎诱导了 SCK-f 克隆中所有细胞的晚期细胞病变效应（CPE）。进一步的测试表明，MRV 在 SCK-f 细胞中达到了 10 50%组织培养感染剂量（TCID）/mL 的峰值病毒滴度，并在九个传代中始终超过 10 TCID/mL。电子显微镜验证了 SCK-f 内的 MRV 病毒粒子完整性。体内实验表明，MRV 感染导致鳜鱼累积死亡率为 86.9%，大口黑鲈（）为 88.9%。这些结果表明 SCK-f 对 MRV 高度允许。本研究强调了细胞多样性在开发病毒允许细胞系中的重要性。SCK 单克隆细胞系池可能为其他鳜鱼病毒生成高度允许的细胞系提供了潜力。
