Rubio Virginia, García-Pérez Ana Isabel, Herráez Angel, Tejedor M Cristina, Diez José C
Departamento de Biología de Sistemas, Unidad de Bioquímica y Biología Molecular, Campus Universitario, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain.
Departamento de Biología de Sistemas, Unidad de Bioquímica y Biología Molecular, Campus Universitario, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain.
Exp Toxicol Pathol. 2017 Oct 2;69(8):700-712. doi: 10.1016/j.etp.2017.08.001. Epub 2017 Sep 7.
Esculetin is a polyphenolic compound with cytoprotective properties. We previously demonstrated the induction of apoptosis by esculetin in NB4 human leukemia cells, as a model, by a mechanism not well understood. To analyse the antioxidant activity of esculetin on apoptosis, we have studied the influence of co-treatments of esculetin at a concentration of 100μM with exogenous ROS donors, namely tert-butyl-hydroperoxide and hydrogen peroxide, on NB4 cells. Esculetin (100μM) exerts a protective effect on cell viability and death necrosis or late apoptosis caused by the oxidant t-BHP whereas it potentiates decrease of cell viability and cell death caused by HO. In the first case, the O scavenging activity of esculetin (100μM) could be implicated. In the last one, cytotoxicity by apoptosis induction seems to be related to the increase in O, among other possible mechanisms. These results contribute to the study of the antitumor properties of esculetin by regulation of redox balance in leukemia cells.
七叶亭是一种具有细胞保护特性的多酚化合物。我们之前以NB4人白血病细胞为模型，证明了七叶亭通过一种尚未完全了解的机制诱导细胞凋亡。为了分析七叶亭对凋亡的抗氧化活性，我们研究了浓度为100μM的七叶亭与外源性活性氧供体（即叔丁基过氧化氢和过氧化氢）共同处理对NB4细胞的影响。七叶亭（100μM）对由氧化剂叔丁基过氧化氢引起的细胞活力、死亡坏死或晚期凋亡具有保护作用，而它会增强由过氧化氢引起的细胞活力下降和细胞死亡。在第一种情况下，七叶亭（100μM）的氧清除活性可能起作用。在最后一种情况下，除其他可能机制外，通过诱导凋亡产生的细胞毒性似乎与氧的增加有关。这些结果有助于通过调节白血病细胞中的氧化还原平衡来研究七叶亭的抗肿瘤特性。
