Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Genomics. 2018 Mar;110(2):124-133. doi: 10.1016/j.ygeno.2017.09.003. Epub 2017 Sep 7.
Agar and agarose have wide applications in food and pharmaceutical industries. Knowledge on the genome of red seaweeds that produce them is still lacking. To fill the gap in genome analyses of these red algae, we have sequenced the nuclear and organellar genomes of an agarophyte, Gracilaria changii. The partial nuclear genome sequence of G. changii has a total length of 35.8Mb with 10,912 predicted protein coding sequences. Only 39.4% predicted proteins were found to have significant matches to protein sequences in SwissProt. The chloroplast genome of G. changii is 183,855bp with a total of 201 open reading frames (ORFs), 29 tRNAs and 3 rRNAs predicted. Five genes: ssrA, leuC and leuD CP76_p173 (orf139) and pbsA were absent in the chloroplast genome of G. changii. The genome information is valuable in accelerating functional studies of individual genes and resolving evolutionary relationship of red seaweeds.
琼胶和琼脂糖在食品和制药工业中有广泛的应用。关于生产它们的红海藻的基因组知识仍然缺乏。为了填补这些红藻基因组分析的空白，我们对一种琼胶植物石花菜进行了核和细胞器基因组测序。G. changii 的部分核基因组序列全长 35.8Mb，预测有 10912 个蛋白编码序列。只有 39.4%的预测蛋白被发现与 SwissProt 中的蛋白序列有显著匹配。G. changii 的叶绿体基因组长 183855bp，共预测了 201 个开放阅读框（ORFs）、29 个 tRNA 和 3 个 rRNA。叶绿体基因组中缺失了五个基因：ssrA、leuC 和 leuD CP76_p173（orf139）和 pbsA。该基因组信息对于加速单个基因的功能研究和解决红海藻的进化关系具有重要价值。
