脂质体包裹的二酰基甘油和肌醇三磷酸诱导卵母细胞延迟激活及孤雌胚发育不良。

Liposome-encapsulated diacyl glycerol and inositol triphosphate-induced delayed oocyte activation and poor development of parthenotes.

作者信息

Nair Ramya, Manikkath Jyothsna, Hegde Aswathi R, Mutalik Srinivas, Kalthur Guruprasad, Adiga Satish Kumar

机构信息

Department of Clinical Embryology, Central Research Lab, Kasturba Medical College, Manipal University, Manipal, India.

Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, India.

出版信息

J Turk Ger Gynecol Assoc. 2017 Sep 1;18(3):102-109. doi: 10.4274/jtgga.2017.0014.

DOI:10.4274/jtgga.2017.0014

PMID:28890423

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5590204/

Abstract

OBJECTIVE

To explore the ability of diacyl glycerol (DAG) and inositol triphosphate (IP3), two major secondary messengers in the calcium signaling pathway, in activating oocytes.

MATERIAL AND METHODS

Oocyte cumulus complex obtained from superovulated Swiss albino mice were incubated in M16 medium with liposome-encapsulated 1,2-Dipalmitoyl-sn-glycerol (LEDAG) and/or IP3 for 3 h. Strontium chloride was used as positive control. The activation potential, ploidy status, and blastocyst rate was calculated.

RESULTS

Both DAG and IP3, individually, induced activation in ~98% of oocytes, which was significantly higher (p<0.01) than activation induced by strontium chloride (60%). Delayed pronucleus formation and a higher percentage of diploid parthenotes was observed in oocytes activated with LEDAG and/or IP3. However, these embryos failed to progress beyond the 6-8-cell stage. Only when the medium was supplemented with LEDAG (5 μg/mL) and IP3 (10 μg/mL) could activated oocytes progress till the blastocyst stage (5.26%), which was lower than the blastocyst rate in the positive controls (13.91%).

CONCLUSION

The results of the present study indicate that DAG and IP3 can induce delayed oocyte activation and poor development of parthenotes in vitro.

摘要

目的

探讨钙信号通路中的两种主要第二信使二酰甘油（DAG）和肌醇三磷酸（IP3）激活卵母细胞的能力。

材料与方法

从超排的瑞士白化小鼠获得的卵母细胞-卵丘复合体在含有脂质体包裹的1,2-二棕榈酰-sn-甘油（LEDAG）和/或IP3的M16培养基中孵育3小时。使用氯化锶作为阳性对照。计算激活潜能、倍性状态和囊胚率。

结果

DAG和IP3单独作用时，均可诱导约98%的卵母细胞激活，这显著高于氯化锶诱导的激活率（60%，p<0.01）。在用LEDAG和/或IP3激活的卵母细胞中观察到原核形成延迟和二倍体孤雌胚比例更高。然而，这些胚胎未能发育到6-8细胞期以后。只有当培养基中添加LEDAG（5μg/mL）和IP3（10μg/mL）时，激活的卵母细胞才能发育到囊胚期（5.26%），这低于阳性对照中的囊胚率（13.91%）。