College of Animal Science and State Key Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100193, People's Republic of China.
Fertil Steril. 2010 Feb;93(3):959-66. doi: 10.1016/j.fertnstert.2008.12.045. Epub 2009 Feb 24.
To investigate the effect of Taxol pretreatment on mitochondrial behaviors in vitrified mouse mature oocytes and their parthenogenetic embryos.
Experimental animal study.
University research laboratory and state key laboratory.
ANIMAL(S): Sexually mature female Kunming white strain mice.
INTERVENTION(S): Taxol before vitrification group (Tax). Oocytes were pretreated with M(2) containing 1 mmol/L Taxol for 2 minutes at 37C and then vitrified-warmed using the OPS vitrification procedure. Both ED solution and EDFS30 solution contained 1 mmol/L Taxol.
MAIN OUTCOME MEASURE(S): Mitochondrial behaviors examined by fluorescence microscopy technology and fluorescence recovery after photobleaching (FRAP) technology.
RESULT(S): In the control group, mitochondria were homogeneously distributed, in slow movement in oocytes, and perinuclearly distributed in 42.6% (n = 115) of their parthenogenetic two-cell embryos. Mitochondria from the toxicity group showed similar localization and movement to those of the control group, but not in the vitrification group. The perinuclear mitochondrial localization pattern of two-cell embryos was statistically significantly lower in both the toxicity (27.2%) and vitrification groups (19.8%) than in the control group. After parthenogenetic activation, the blastocyst formation rate of oocytes in the treated groups (28.1 to 48.6%) was statistically significantly lower than that of control (61.2%), but the rate of Taxol group (47.9%) was statistically significantly higher than that in the vitrification group (28.1%).
CONCLUSION(S): Taxol pretreatment before vitrification helps to reduce the mitochondrial disturbance induced by vitrification in oocytes and their parthenogenetic early-stage embryo.
探讨紫杉醇预处理对玻璃化冷冻小鼠成熟卵母细胞及其孤雌胚胎中线粒体行为的影响。
实验动物研究。
大学研究实验室和国家重点实验室。
性成熟昆明白色品系雌性小鼠。
紫杉醇预处理组（Tax）。卵母细胞在 37°C 下用含 1mmol/L 紫杉醇的 M（2）预处理 2 分钟，然后用 OPS 玻璃化程序进行玻璃化-解冻。ED 溶液和 EDFS30 溶液均含有 1mmol/L 紫杉醇。
荧光显微镜技术和荧光恢复后漂白（FRAP）技术检测线粒体行为。
在对照组中，线粒体均匀分布，在卵母细胞中缓慢移动，在 42.6%（n=115）的孤雌二细胞胚胎中呈核周分布。毒性组的线粒体表现出与对照组相似的定位和运动，但在玻璃化组中没有。在毒性组（27.2%）和玻璃化组（19.8%）中，两细胞胚胎核周线粒体定位模式均明显低于对照组。经孤雌激活后，处理组卵母细胞的囊胚形成率（28.1%至 48.6%）明显低于对照组（61.2%），但紫杉醇组（47.9%）明显高于玻璃化组（28.1%）。
玻璃化冷冻前紫杉醇预处理有助于减少卵母细胞及其孤雌早期胚胎玻璃化引起的线粒体紊乱。
