Cheng Po-Liang, Wu Hui-Ru, Li Cheng-Yan, Chen Chih-Feng, Cheng Hsu-Chen
Center for integrative Evolutionary Galliform Genomics Research (iEGG Center), National Chung Hsing University, Taichung City 402, Taiwan.
Department of Life Sciences, National Chung Hsing University, Taichung City 402, Taiwan.
J Reprod Dev. 2017 Dec 15;63(6):563-570. doi: 10.1262/jrd.2017-090. Epub 2017 Sep 8.
Previous studies have shown that grafted neonatal chicken testicular tissue can develop and produce functional sperm; however, it was unclear whether regenerative processes or proportional growth caused the re-appearance of spermatogenic tissue. We dissociated testicular tissues, performed subcutaneous auto-transplantation of the re-aggregated cells to castrated cockerels, and monitored the post-surgery development of these transplanted aggregates. We found that these transplanted cell aggregates experienced compensatory growth in the form of a 300-fold increase in size, rather than the 30-fold increase observed in normal testis development. Further, these dissociated testicular cell aggregates restored seminiferous tubule structure and were able to produce testosterone and motile sperm. Therefore, we concluded that the dissociated testicular cells from 11-week-old cockerels retained a strong regenerative potential, as they exhibited compensatory growth, restored destroyed structure, and sustained spermatogenesis.
先前的研究表明，移植的新生鸡睾丸组织能够发育并产生功能性精子；然而，尚不清楚是再生过程还是比例性生长导致了生精组织的重新出现。我们将睾丸组织解离，对重新聚集的细胞进行皮下自体移植到去势公鸡体内，并监测这些移植聚集体术后的发育情况。我们发现，这些移植的细胞聚集体经历了代偿性生长，大小增加了300倍，而不是正常睾丸发育中观察到的30倍增加。此外，这些解离的睾丸细胞聚集体恢复了生精小管结构，并能够产生睾酮和活动精子。因此，我们得出结论，11周龄公鸡解离的睾丸细胞保留了强大的再生潜力，因为它们表现出代偿性生长、恢复了被破坏的结构并维持了精子发生。
