Chiavaroli A, Recinella L, Ferrante C, Martinotti S, Vacca M, Brunetti L, Orlando G, Leone S
Department of Pharmacy, “G. d’Annunzio” University, Chieti, Italy
J Biol Regul Homeost Agents. 2017 Jul-Sep;31(3):797-802.
Adipose tissue and skeletal muscle are organs capable of secreting many bioactive molecules, such as adipomiokines that could be possibly involved in mood disorders. In the present work, we investigated the possible behavioral effects of a single intracerebroventricular (i.c.v.) injection of two adipomiokines, fibrobroblast growth factor (FGF)-21 (0.5-5.0 µg) and irisin (0.4-0.6 µg), in male rats tested in the open field and elevated plus maze tests. Prefrontal cortex levels of norepinephrine (NE), dopamine (DA) and serotonin (5-hydroxytryptamine, 5-HT) and the gene expression of catechol-O-methyltransferase (COMT), dopamine transport (DAT) and tyrosine hydroxylase (TH), were measured by high performance liquid chromatography (HPLC) analysis and real-time reverse transcription polymerase chain reaction (RT-PCR). Both FGF-21 and irisin administration induced anxiogenic behavior, increased DA levels in prefrontal cortex, decreased COMT, DAT and increased TH gene expression. In conclusion, in the present study we demonstrated behavioral effects induced by central FGF-21 and irisin injections that could involve increased DA signaling in the prefrontal cortex.
脂肪组织和骨骼肌是能够分泌多种生物活性分子的器官，比如可能与情绪障碍有关的脂肪因子。在本研究中，我们调查了在旷场试验和高架十字迷宫试验中，向雄性大鼠脑室内（i.c.v.）单次注射两种脂肪因子，即成纤维细胞生长因子（FGF）-21（0.5 - 5.0微克）和鸢尾素（0.4 - 0.6微克）后可能产生的行为学效应。通过高效液相色谱（HPLC）分析和实时逆转录聚合酶链反应（RT-PCR）测量前额叶皮质中去甲肾上腺素（NE）、多巴胺（DA）和5-羟色胺（5-HT）的水平以及儿茶酚-O-甲基转移酶（COMT）、多巴胺转运体（DAT）和酪氨酸羟化酶（TH）的基因表达。给予FGF-21和鸢尾素均诱发了焦虑行为，增加了前额叶皮质中的DA水平，降低了COMT、DAT水平并增加了TH基因表达。总之，在本研究中我们证明了脑室内注射FGF-21和鸢尾素所诱发的行为学效应，这可能涉及前额叶皮质中DA信号的增强。
