Mohamed Harir, Miloud Bellahcene, Zohra Fortas, García-Arenzana José María, Veloso Antonio, Rodríguez-Couto Susana
Biology of Microorganisms and Biotechnology Laboratory, University of Oran 1 Ahmed Ben Bella, Oran, Algeria.
Institut of Sciences, Faculty of Sciences, Natural and Life Sciences Department, University Center of Ain Temouchent, Temouchent, Algeria.
Int J Mol Cell Med. 2017 Spring;6(2):109-120. doi: 10.22088/acadpub.BUMS.6.2.5. Epub 2017 Jul 11.
Extreme ecosystems can be a source of untapped microorganisms to produce novel bioactive compounds of industrial interest. Consequently, in this work, 32 actinomycetes were isolated from 6 soil samples collected from Algerian Sahara in searching for untapped producers of novel antimicrobial compounds. All the isolates were further subjected to antimicrobial screening against pathogenic bacteria, yeast and fungi. The obtained results indicated that three of the isolates (named C, MS1 and 10) showed antimicrobial activities against most of the tested pathogenic microorganisms. Therefore, these three promising isolates, previously identified as by morphological, biochemical and physiological methods, were selected for their subsequent identification by the whole cell matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) analysis. Thus, the isolates C, MS1 and 10 were identified as B263 UFL, B262 UFL and B192 UFL, respectively. These results pointed out actinomycetes from Sahara soils as potential sources of novel antimicrobial compounds. Also, MALDI-TOF MS showed to be a robust technique for bacteria identification.
极端生态系统可能是未开发微生物的来源，这些微生物可产生具有工业价值的新型生物活性化合物。因此，在本研究中，从阿尔及利亚撒哈拉地区采集的6份土壤样本中分离出32株放线菌，以寻找新型抗菌化合物的未开发生产者。所有分离株进一步针对病原菌、酵母和真菌进行抗菌筛选。所得结果表明，其中三株分离株（命名为C、MS1和10）对大多数测试病原菌表现出抗菌活性。因此，这三株有前景的分离株，之前已通过形态学、生化和生理学方法鉴定，因其后续通过全细胞基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）分析进行鉴定而被选中。因此，分离株C、MS1和10分别被鉴定为B263 UFL、B262 UFL和B192 UFL。这些结果指出撒哈拉土壤中的放线菌是新型抗菌化合物的潜在来源。此外，MALDI-TOF MS显示是一种用于细菌鉴定的强大技术。
