El Sayed Osama H, Asker Mohsen M S, Swelim Mahmoud A, Abbas Ibrahim H, Attwa Aziza I, El Awady Mohamed E
Department of Microbial Biotechnology, National Research Centre, 33 Bohouth St., Dokki, Giza 12622, Egypt.
Botany Department, Faculty of Science, Benha University, Benha, Egypt.
J Genet Eng Biotechnol. 2016 Jun;14(1):161-168. doi: 10.1016/j.jgeb.2016.04.001. Epub 2016 May 12.
Hydroxy marilone C is a bioactive metabolite produced from the culture broth of isolated from Egyptian soil. Hydroxy marilone C was purified and fractionated by a silica gel column with a gradient mobile phase dichloromethane (DCM):methanol then Sephadex LH-20 column using methanol as a mobile phase. It was subjected to many procedures such as infrared (IR), nuclear magnetic resonance (NMR), Mass spectroscopy (MS) and UV spectroscopy for elucidation of its structure. It was evaluated for antioxidant, cytotoxicity against human alveolar basal epithelial cell line (A-549) and human breast adenocarcinoma cell line (MCF-7) and antiviral activities; showed that the maximum antioxidant activity was 78.8% at 3 mg/ml after 90 min. and the IC value against DPPH radical found about 1.5 mg/ml after 60 min. Using MTT assay the effect of the pure compound on the proliferation of A-549 cells and MCF-7 cells was 443 μg/ml and 147.9 μg/ml, respectively, while for detection of antiviral activity using Madin-Darby canine kidney (MDCK) cells the maximum cytotoxicity was at 27.9% and IC was 128.1 μg/ml. The maximum concentration required for protecting 50% of the virus-infected cells against H1N1 viral cytopathogenicity (EC) was 33.25% for 80 μg/ml. These results indicated that the hydroxy marilone C has potential antitumor and antiviral activities.
羟基马里酮C是从埃及土壤中分离出的菌株的培养液中产生的一种生物活性代谢产物。羟基马里酮C通过硅胶柱进行纯化和分级分离，流动相采用二氯甲烷（DCM）：甲醇梯度洗脱，然后使用甲醇作为流动相通过葡聚糖LH - 20柱进行分离。它经过了许多程序，如红外（IR）、核磁共振（NMR）、质谱（MS）和紫外光谱分析以阐明其结构。对其抗氧化、对人肺泡基底上皮细胞系（A - 549）和人乳腺腺癌细胞系（MCF - 7）的细胞毒性以及抗病毒活性进行了评估；结果表明，在90分钟后，3mg/ml时的最大抗氧化活性为78.8%，60分钟后对DPPH自由基的IC值约为1.5mg/ml。使用MTT法，该纯化合物对A - 549细胞和MCF - 7细胞增殖的影响分别为443μg/ml和147.9μg/ml，而使用麦迪逊 - 达比犬肾（MDCK）细胞检测抗病毒活性时，最大细胞毒性为27.9%，IC值为128.1μg/ml。保护50%的病毒感染细胞免受H1N1病毒细胞病变效应（EC）所需的最大浓度，对于80μg/ml的药物为33.25%。这些结果表明羟基马里酮C具有潜在的抗肿瘤和抗病毒活性。
