Nitsche Christoph, Mahawaththa Mithun C, Becker Walter, Huber Thomas, Otting Gottfried
Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia.
Chem Commun (Camb). 2017 Oct 3;53(79):10894-10897. doi: 10.1039/c7cc06155b.
Site-selective chemical protein modification is achieved by self-assembly of a specific di-cysteine motif, trivalent pnictogens (As, Sb or Bi) and an aromatic mercaptomethyl-based probe. The strategy is demonstrated with a quaternary complex involving Zika virus protease and a lanthanide ion, enabling paramagnetic nuclear magnetic resonance spectroscopy and luminescence measurements.
通过特定二硫氨酸基序、三价氮族元素（砷、锑或铋）和基于芳基巯基甲基的探针的自组装实现位点选择性化学蛋白质修饰。该策略通过涉及寨卡病毒蛋白酶和镧系离子的四元复合物得以证明，从而实现顺磁核磁共振光谱和发光测量。
