通过氮族元素介导的自组装对蛋白质进行位点选择性标记
Site-selective tagging of proteins by pnictogen-mediated self-assembly.
作者信息
Nitsche Christoph, Mahawaththa Mithun C, Becker Walter, Huber Thomas, Otting Gottfried
机构信息
Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia.
出版信息
Chem Commun (Camb). 2017 Oct 3;53(79):10894-10897. doi: 10.1039/c7cc06155b.
PMID:28890978
Abstract
Site-selective chemical protein modification is achieved by self-assembly of a specific di-cysteine motif, trivalent pnictogens (As, Sb or Bi) and an aromatic mercaptomethyl-based probe. The strategy is demonstrated with a quaternary complex involving Zika virus protease and a lanthanide ion, enabling paramagnetic nuclear magnetic resonance spectroscopy and luminescence measurements.
摘要
通过特定二硫氨酸基序、三价氮族元素（砷、锑或铋）和基于芳基巯基甲基的探针的自组装实现位点选择性化学蛋白质修饰。该策略通过涉及寨卡病毒蛋白酶和镧系离子的四元复合物得以证明，从而实现顺磁核磁共振光谱和发光测量。
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