Nesthus I, Glette J, Bjorvatn B, Solberg C O
Medical Department B, Haukeland sykehus, Bergen, Norway.
NIPH Ann. 1987 Jun;10(1):11-9.
The tube migration test for leukocyte function studies has been modified to test the motility of Entamoeba histolytica. The test was more sensitive to the effect of antiamebic drugs than was a test for phagocytic activity. A 50% reduction in amebic motility was seen instantly after exposure to metronidazole (40 mg/l). A similar reduction of phagocytosis at the same concentration of the drug was seen only after two hours' preexposure. The extent of amebic migration in the test tube is dependent on factors such as concentration of parasites, temperature and incubation time. The tube migration test is cheap and simple and could be a valuable supplement to existing screening methods for potential antiamebic drugs.
用于白细胞功能研究的试管迁移试验已被改进，以测试溶组织内阿米巴的运动能力。该试验对抗阿米巴药物的作用比吞噬活性试验更为敏感。暴露于甲硝唑（40毫克/升）后，立即观察到阿米巴运动能力降低了50%。在相同药物浓度下，仅在预先暴露两小时后才观察到吞噬作用有类似程度的降低。试管中阿米巴的迁移程度取决于寄生虫浓度、温度和孵育时间等因素。试管迁移试验成本低廉且操作简单，可为现有潜在抗阿米巴药物筛选方法提供有价值的补充。
