Albery W J, Bartlett P N, Cass A E
Department of Chemistry, Imperial College of Science and Technology, London, U.K.
Philos Trans R Soc Lond B Biol Sci. 1987 Aug 28;316(1176):107-19. doi: 10.1098/rstb.1987.0021.
Three different types of amperometric enzyme electrode are described. The first type uses a conducting organic-salt electrode to oxidize NADH. Results for sensors for ethanol and for bile acids are presented. In the second type of sensor, flavoenzymes are directly oxidized on the surface of the conducting organic-salt electrode. Results for five different enzymes are described. The mechanism of the enzyme oxidation is discussed and the reaction is shown to take place by heterogeneous redox catalysis and not by homogeneous mediation. The enzymes are strongly adsorbed on the electrode; microelectrodes for in vivo studies can be constructed without a membrane. Results for in vivo studies of changing glucose levels in the brain of a freely moving rat are presented. The third type of sensor is designed to measure low levels of toxic gases such as H2S and HCN. This is done by monitoring the inhibition by the toxic gas of the activity of the respiratory enzyme cytochrome oxidase.
本文描述了三种不同类型的电流型酶电极。第一种类型使用导电有机盐电极来氧化NADH。文中给出了乙醇传感器和胆汁酸传感器的相关结果。在第二种类型的传感器中，黄素酶在导电有机盐电极表面被直接氧化。文中描述了五种不同酶的相关结果。本文讨论了酶氧化的机制，并表明该反应是通过非均相氧化还原催化发生的，而不是通过均相介导。这些酶被强烈吸附在电极上；可以构建无膜的用于体内研究的微电极。文中给出了在自由活动大鼠大脑中葡萄糖水平变化的体内研究结果。第三种类型的传感器旨在测量低水平的有毒气体，如H2S和HCN。这是通过监测有毒气体对呼吸酶细胞色素氧化酶活性的抑制作用来实现的。
