Li Zhengwei, Wang Ying
Department of Pediatric Surgery, First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang 471003, Henan, China.
Biochem Cell Biol. 2018 Jun;96(3):365-371. doi: 10.1139/bcb-2017-0183. Epub 2017 Sep 11.
Recent research suggested that microRNA 96 (miR-96) might function as an oncogene in several types of cancers. Therefore, the purpose of this study was to probe into the mechanism of miR-96 in hepatocellular carcinoma (HCC) cells. HCC tissues and non-tumorous tissues, HCC cell lines, and healthy cell lines were all involved in this study. Quantitative real-time PCR (qRT-PCR) and Western blot were used to detect miR-96 and SOX6 mRNA and protein expressions. The direct regulation of miR96 on SOX6 was confirmed by luciferase reporter assays. Cell proliferation and growth were determined by MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) assay and colony formation assay. Wound healing and transwell assay were employed for migration and invasion analyses. Finally, SPSS 21.0 and GraphPad 7.0 were applied for statistical analyses. In HCC tissues, miR-96 was highly expressed while SOX6 was lowly expressed. The overexpression of miR-96 reversely inhibited the expression of SOX6, contributing to the promotion of the biological functions of HCC cells. miR-96 could promote cell proliferation, migration, and invasion in HCC by targeting SOX6.
近期研究表明，微小RNA 96（miR-96）可能在多种癌症中发挥癌基因的作用。因此，本研究旨在探究miR-96在肝癌（HCC）细胞中的作用机制。本研究纳入了HCC组织和非肿瘤组织、HCC细胞系以及健康细胞系。采用定量实时聚合酶链反应（qRT-PCR）和蛋白质免疫印迹法检测miR-96和SOX6 mRNA及蛋白表达。荧光素酶报告基因检测证实了miR-96对SOX6的直接调控作用。通过MTT（3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2-H-四氮唑溴盐）法和集落形成试验测定细胞增殖和生长情况。采用伤口愈合试验和Transwell试验进行迁移和侵袭分析。最后，应用SPSS 21.0和GraphPad 7.0进行统计分析。在HCC组织中，miR-96高表达而SOX6低表达。miR-96的过表达反向抑制SOX6的表达，促进了HCC细胞的生物学功能。miR-96可通过靶向SOX6促进HCC细胞的增殖、迁移和侵袭。
