Ledley F D, Grenett H E, Bartos D P, van Tuinen P, Ledbetter D H, Woo S L
Howard Hughes Medical Institute, Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
Somat Cell Mol Genet. 1987 Sep;13(5):575-80. doi: 10.1007/BF01534499.
A cDNA clone for rabbit tryptophan hydroxylase was used as a probe to identify human tryptophan hydroxylase gene fragments in a panel of hamster-human somatic cell hybrids and determine its chromosomal location in man. A single locus was identified for tryptophan hydroxylase on chromosome 11. Tryptophan hydroxylase is a member of the superfamily of pterin-dependent aromatic amino acid hydroxylases which includes tyrosine hydroxylase, located at 11p15.5-p15, and phenylalanine hydroxylase, located at 12q22-q24.1 in human. The locations of these genes and the evolutionary distance between their sequences suggest that at least three distinct genetic events have occurred during the evolution of the aromatic amino acid hydroxylase superfamily: two sequential gene duplications giving rise to the three distinct hydroxylase loci, and a translocation which separated the tryptophan and tyrosine hydroxylase loci on chromosome 11 from the phenylalanine hydroxylase locus on chromosome 12.
用兔色氨酸羟化酶的一个cDNA克隆作为探针，在一组仓鼠-人类体细胞杂种中鉴定人类色氨酸羟化酶基因片段，并确定其在人类中的染色体定位。在11号染色体上鉴定出色氨酸羟化酶的一个单一位点。色氨酸羟化酶是蝶呤依赖性芳香族氨基酸羟化酶超家族的成员，该超家族包括位于11p15.5-p15的酪氨酸羟化酶和位于人类12q22-q24.1的苯丙氨酸羟化酶。这些基因的位置及其序列之间的进化距离表明，在芳香族氨基酸羟化酶超家族的进化过程中至少发生了三个不同的遗传事件：两次连续的基因复制产生了三个不同的羟化酶位点，以及一次易位，将11号染色体上的色氨酸和酪氨酸羟化酶位点与12号染色体上的苯丙氨酸羟化酶位点分开。
