Effectivity of Titanium Oxide Based Nano Particles on from Clinical Samples.

INTRODUCTION

Nanoparticles composed of Titanium Oxide (TiO) are non toxic, durable, stable and have a high refractive index with a lot of scope in biomedical applications. Due to their antibacterial effects, they can be applied to inanimate objects like glass, metal and even biomedical implants.

AIM

This study was conducted to assess the antibacterial effect of Titanium Oxide (TiO) alone or with Silver (Ag) as an additive on

MATERIALS AND METHODS

isolates (n=25) sensitive to most of the drugs including first generation cephalosporins, ampicillin and amoxycillin from various samples like pus, urine, sputum and blood were placed onto the glass slides containing TiO annealed at 200°C, 400°C, TiO with 0.1% Ag as additive, TiO with 0.3% Ag, and TiO with 0.6% Ag as additive. Samples from this were inoculated at every hour onto sterile petri plates and observed for growth after overnight incubation at 37°C.

RESULTS

The organisms which were inoculated onto TiO annealed at 200°C showed a slower reduction rate from >1 × 108 cfu/ml to <1 × 10 cfu/ml only after six hours of incubation in visible light. Complete absence of colony forming units was observed after eight hours of incubation. The samples treated with TiO at 400°C showed no growth after six hours of incubation itself. Samples treated with TiO with increasing gradations of silver as additives showed proportional reduction in the incubation time for the complete absence of colony forming units.

CONCLUSION

Our study shows that pure titanium oxide has a high antibacterial effect on pathogenic samples of from clinical isolates, which is further increased with the addition of increasing concentrations of silver.