Sansoni P, Rowden G, Manara G C, Ferrari C, De Panfilis G
Department of Medicine, University of Parma, Italy.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1987;53(5):301-7. doi: 10.1007/BF02890256.
The present report concerns the demonstration of the exclusive detection among peripheral blood T-lymphocytes of the S-100 protein within the CD8-positive subpopulation which lacks the antigen recognized by the 9.3 monoclonal antibody. Highly purified human peripheral blood T-cell subsets, obtained by means of panning techniques, were first stained, by an immunofluorescence method, with purified anti-S-100 protein antibodies. The vast majority of S-100 protein- (and, specifically, its beta subunit) positive cells were detected in the CD8-positive, 9.3-negative subset. This subset had previously been shown to comprise all the alloantigen-specific and histamine-inducible suppressor T-cells. Other T-subsets, even those showing either CD8-positivity (but 9.3-positivity) or 9.3-negativity (but CD8-negativity), were, as a rule, S-100 negative. Immunoelectronmicroscopy confirmed that the S-100-positive cells, showing peroxidase activity within the cytoplasm, were found exclusively within the CD8-positive, 9.3-negative subset. This finding of S-100 protein in cells of a specific T8 suppressor subset extends the range of the known distribution of this protein and may have important implications concerning its role in the modulation of immune responses.
本报告涉及在缺乏9.3单克隆抗体所识别抗原的CD8阳性亚群外周血T淋巴细胞中独家检测S-100蛋白。通过淘选技术获得的高度纯化的人外周血T细胞亚群，首先用免疫荧光法用纯化的抗S-100蛋白抗体染色。绝大多数S-100蛋白（特别是其β亚基）阳性细胞在CD8阳性、9.3阴性亚群中被检测到。该亚群先前已被证明包含所有同种异体抗原特异性和组胺诱导性抑制性T细胞。其他T亚群，即使是那些显示CD8阳性（但9.3阳性）或9.3阴性（但CD8阴性）的亚群，通常也是S-100阴性。免疫电子显微镜证实，在细胞质内显示过氧化物酶活性的S-100阳性细胞仅在CD8阳性、9.3阴性亚群中发现。在特定T8抑制亚群细胞中发现S-100蛋白这一结果扩展了该蛋白已知分布范围，可能对其在免疫反应调节中的作用具有重要意义。
