Alborali Giovanni Loris, Ruggeri Jessica, Pesciaroli Michele, Martinelli Nicola, Chirullo Barbara, Ammendola Serena, Battistoni Andrea, Ossiprandi Maria Cristina, Corradi Attilio, Pasquali Paolo
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna [Experimental Zooprophylactic Institute of Lombardy and Emilia Romagna], 25124, Brescia, Italy.
FAO Reference Center for Veterinary Public Health, Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, 00161, Rome, Italy.
BMC Vet Res. 2017 Sep 11;13(1):284. doi: 10.1186/s12917-017-1202-5.
Salmonella enterica serovar Choleraesuis (S. Choleraesuis) infection causes a systemic disease in pigs. Vaccination could represent a solution to reduce prevalence in farms. In this study, we aimed to assess the efficacy of an attenuated strain of Salmonella enterica serovar Typhimurium (S. Typhimurium ΔznuABC) against S. Choleraesuis infection. The vaccination protocol combined priming with attenuated S. Typhimurium ΔznuABC vaccine and boost with an inactivated S. Choleraesuis vaccine and we compared the protection conferred to that induced by an inactivated S. Choleraesuis vaccine.
The first group of piglets was orally vaccinated with S. Typhimurium ΔznuABC and boosted with inactivated S. Choleraesuis, the second one was intramuscularly vaccinated with S. Choleraesuis inactivated vaccine and the third group of piglets was unvaccinated. All groups of animals were challenged with a virulent S. Choleraesuis strain at day 35 post vaccination.
The results showed that the vaccination protocol, priming with S. Typhimurium ΔznuABC and boosted with inactivated S. Choleraesuis, applied to group A was able to limit weight loss, fever and organs colonization, arising from infection with virulent S. Choleraesuis, more effectively, than the prime-boost vaccination with homologous S. Choleraesuis inactivated vaccine (group B).
In conclusion, these research findings extend the validity of attenuated S. Typhimurium ΔznuABC strain as a useful mucosal vaccine against S. Typhimurium and S. Choleraesuis pig infection. The development of combined vaccination protocols can have a diffuse administration in field conditions because animals are generally infected with different concomitant serovars.
猪霍乱沙门氏菌（S. Choleraesuis）感染可导致猪的全身性疾病。接种疫苗可能是降低猪场发病率的一种解决办法。在本研究中，我们旨在评估鼠伤寒沙门氏菌减毒株（S. Typhimurium ΔznuABC）对猪霍乱沙门氏菌感染的疗效。疫苗接种方案为先使用鼠伤寒沙门氏菌ΔznuABC减毒疫苗进行初次免疫，再使用猪霍乱沙门氏菌灭活疫苗进行加强免疫，我们将其诱导的保护作用与猪霍乱沙门氏菌灭活疫苗诱导的保护作用进行了比较。
第一组仔猪口服接种鼠伤寒沙门氏菌ΔznuABC，并用猪霍乱沙门氏菌灭活疫苗进行加强免疫；第二组仔猪肌肉注射猪霍乱沙门氏菌灭活疫苗；第三组仔猪不接种疫苗。所有动物组在接种疫苗后第35天用强毒猪霍乱沙门氏菌菌株进行攻毒。
结果表明，应用于A组的先使用鼠伤寒沙门氏菌ΔznuABC进行初次免疫、再使用猪霍乱沙门氏菌灭活疫苗进行加强免疫的疫苗接种方案，比使用同源猪霍乱沙门氏菌灭活疫苗进行初次免疫-加强免疫（B组）更能有效限制由强毒猪霍乱沙门氏菌感染引起的体重减轻、发热和器官定植。
总之，这些研究结果扩展了鼠伤寒沙门氏菌ΔznuABC减毒株作为一种有效的黏膜疫苗预防鼠伤寒沙门氏菌和猪霍乱沙门氏菌感染猪的有效性。联合疫苗接种方案的开发在野外条件下可以广泛应用，因为动物通常会感染不同的伴随血清型。
