Foss Dennis L, Agin Tonia S, Bade Donald, Dearwester Don A, Jolie Rika, Keich Robin L, Lohse Robert M, Reed Maryke, Rosey Everett L, Schneider Patricia A, Taylor Lucas P, Willy Michael S
Zoetis Inc., 333 Portage Street, Kalamazoo, MI 49007, USA.
Vet Immunol Immunopathol. 2013 Sep 1;155(1-2):76-86. doi: 10.1016/j.vetimm.2013.06.007. Epub 2013 Jun 14.
Pre-harvest reduction of Salmonella carriage by swine would benefit both animal health and food quality. While vaccination is an attractive pre-harvest intervention to reduce Salmonella levels in swine, the large number of potential Salmonella enterica serovars found in swine makes it critical that vaccines provide broad serotype efficacy. In order to directly compare the relative efficacy of Salmonella vaccines against serogroup-matched and serogroup-unmatched Salmonella, we vaccinated pigs with two commercially available Salmonella vaccines (either serogroup B or serogroup C1) and challenged with serovar-matched, serogroup-matched or serogroup-unmatched challenge strains. We found that while serogroup-matched vaccines provided relatively better efficacy than unmatched vaccines, serotype-unmatched vaccines also provided significant reduction of Salmonella carriage and shed. In addition, by measuring serogroup specific cell mediated (IFN-γ ELISPOT) and humoral (anti-LPS ELISA) immunity, we found that this serogroup specific efficacy correlates primarily with humoral immunity, while cell mediated immunity was mostly non-serogroup specific. While the practical relevance to pork quality of this serogroup-specific efficacy remains to be demonstrated, the large predominance of serogroup B Salmonella in swine suggests that a serogroup B Salmonella vaccine for swine would be of value to pre-harvest food safety interventions in swine.
猪在收获前减少沙门氏菌携带量对动物健康和食品质量都有益处。虽然疫苗接种是一种有吸引力的收获前干预措施，可降低猪体内的沙门氏菌水平，但猪体内发现的大量潜在肠炎沙门氏菌血清型使得疫苗具有广泛的血清型效力至关重要。为了直接比较沙门氏菌疫苗对血清群匹配和血清群不匹配的沙门氏菌的相对效力，我们用两种市售沙门氏菌疫苗（血清群B或血清群C1）对猪进行接种，并分别用血清型匹配、血清群匹配或血清群不匹配的攻击菌株进行攻毒。我们发现，虽然血清群匹配的疫苗比不匹配的疫苗提供了相对更好的效力，但血清型不匹配的疫苗也能显著降低沙门氏菌的携带量和排泄量。此外，通过测量血清群特异性细胞介导（IFN-γ ELISPOT）和体液（抗LPS ELISA）免疫，我们发现这种血清群特异性效力主要与体液免疫相关，而细胞介导免疫大多是非血清群特异性的。虽然这种血清群特异性效力对猪肉质量的实际相关性仍有待证明，但猪体内血清群B沙门氏菌的大量占比表明，一种用于猪的血清群B沙门氏菌疫苗对猪收获前食品安全干预具有价值。
