通过白细胞黏附抑制微量测定法检测牛眼鳞状细胞癌中的肿瘤相关免疫。

Tumor-associated immunity in bovine ocular squamous cell carcinoma detected by leukocyte adherence inhibition microassay.

作者信息

Jennings P A, Halliday W J, Hoffmann D

出版信息

J Natl Cancer Inst. 1979 Sep;63(3):775-9. doi: 10.1093/jnci/63.3.775.

DOI:10.1093/jnci/63.3.775

Abstract

A microplate modification of the leukocyte adherence inhibition (LAI) assay was used with blood leukocytes from cattle with ocular squamous cell carcinomas (OSCC); control groups were cattle with ocular or cutaneous lesions (not carcinoma) and healthy normal cattle. For the assay, saline extracts of OSCC and skin from the same donor, lymphosarcoma, and mastocytoma (M) were used as antigens. Specific LAI reactivity to OSCC extract (but not to skin extract) was detected in 14 of 18 animals with squamous cell carcinoma. One animal with OSCC showed LAI reactivity to OSCC extract, but 1 clinically normal cow had LAI with the M-extract. Leukocyte adherence stimulation reactions with the various antigens were seen in all groups of animals.

摘要

采用白细胞黏附抑制（LAI）试验的微孔板改良法，对患有眼鳞状细胞癌（OSCC）的牛的血液白细胞进行检测；对照组为患有眼部或皮肤病变（非癌）的牛以及健康正常的牛。在该试验中，将来自同一供体的OSCC和皮肤的盐水提取物、淋巴肉瘤和肥大细胞瘤（M）用作抗原。在18只患有鳞状细胞癌的动物中，有14只检测到对OSCC提取物（而非皮肤提取物）的特异性LAI反应。1只患有OSCC的动物对OSCC提取物表现出LAI反应，但1只临床正常的母牛对M提取物有LAI反应。在所有动物组中均观察到了与各种抗原的白细胞黏附刺激反应。