Proctor E M, Wong Q, Yang J, Keystone J S
Division of Laboratories, British Columbia Centre for Disease Control, National Reference Service for Parasitology (Morphology), Vancouver, Canada.
Am J Trop Med Hyg. 1987 Sep;37(2):296-301. doi: 10.4269/ajtmh.1987.37.296.
The isoenzyme patterns of 92 isolates of Entamoeba histolytica from British Columbia and 28 from Ontario were determined. Seropositivity for E. histolytica was assessed by indirect hemagglutination and enzyme-linked immunosorbent assay in the one center and by ELISA and amebic gel diffusion in the other. In both British Columbia and Ontario nonpathogenic zymodemes I and III were most common. A newly described isoenzyme pattern was identified in Ontario. Only 9 of 120 zymodeme patterns identified were found to be pathogenic strains of E. histolytica. Pathogenic isolates were strongly correlated with clinical symptoms and seropositivity.
对来自不列颠哥伦比亚省的92株溶组织内阿米巴分离株和来自安大略省的28株分离株的同工酶模式进行了测定。在一个中心通过间接血凝试验和酶联免疫吸附测定法评估溶组织内阿米巴的血清阳性率，在另一个中心则通过酶联免疫吸附测定法和阿米巴凝胶扩散法进行评估。在不列颠哥伦比亚省和安大略省，非致病性酶谱型I和III最为常见。在安大略省发现了一种新描述的同工酶模式。在鉴定出的120种酶谱型中，仅发现9种是溶组织内阿米巴的致病菌株。致病分离株与临床症状和血清阳性率密切相关。
