Kvetnoĭ I M, Iuzhakov V V
Arkh Patol. 1987;49(7):77-80.
A rationale for sequential immunohistochemical studies of the endocrine function of APUD cells is described, as exemplified by a study of serotonin-containing cells in rat small intestine, involving preparation of paraffin and semithin sections of epoxide resin-embedded tissue, followed by electron-microscopic examination of cells detected by the immunoperoxidase technique. With the approach described, it has been shown that a positive immunohistochemical reaction to antiserotonin antiserum is given, along with EC cells, by those mast cells disposed in groups near the EC cells as well as among connective-tissue cells of villi under the basement membrane. It is suggested that by collecting and accumulating the serotonin synthesized by specialized EC cells, mast cells transport it to sites of utilization as required by the specific arrangement.
本文描述了对APUD细胞内分泌功能进行系列免疫组织化学研究的基本原理，以大鼠小肠中含5-羟色胺细胞的研究为例，该研究包括制备环氧树脂包埋组织的石蜡切片和半薄切片，然后对通过免疫过氧化物酶技术检测到的细胞进行电子显微镜检查。采用上述方法已表明，除了肠嗜铬（EC）细胞外，位于EC细胞附近成群分布的肥大细胞以及基底膜下绒毛结缔组织细胞中也会出现抗5-羟色胺抗血清的阳性免疫组织化学反应。有人提出，肥大细胞通过收集和积累由特殊的EC细胞合成的5-羟色胺，并根据特定排列将其输送到利用部位。
