Nogueira T de O, Aubin J E
School of Dentistry of São José dos Campos, São Paulo, UNESP, Brazil.
Histol Histopathol. 1997 Oct;12(4):919-23.
Immunohistochemical screening for monoclonal antibodies prepared by immunization of mice with a rat osteoblastic cell population led to identification of one antibody that reacted against a small population of cells present in the soft connective tissue compartment of 21 days fetal rat calvaria. The morphology of the cells and the immunohistochemical staining characteristics (a distinct intracellular granular pattern) suggested that the antibody might be reacting specifically against mast cells. We used combined histochemistry and immunohistochemistry to further characterize this antibody, designated RCJ102. Cryosections containing calvaria bone, soft connective tissues and skin were prepared from the top of the head of 21 days fetal rats, and from adult rats cryosections of lung, muscle, adipose tissue and small intestine were prepared. Some sections were labelled by indirect immunofluorescence with RCJ102; corresponding sections were labelled histochemically with toluidine blue. There was a direct correspondence between mast cells identified histochemically and cells labelling with RCJ102 in all tissues except intestine, in which the mast cell detectable by histochemistry were not labelled by RCJ102. These results suggest that the RCJ102 antibody will be a valuable new reagent for further elucidation of the heterogeneity described between connective tissue and intestinal mucosal mast cells.
用大鼠成骨细胞群体免疫小鼠制备单克隆抗体，通过免疫组织化学筛选，鉴定出一种抗体，该抗体可与21天龄胎鼠颅骨软结缔组织区中少量细胞发生反应。细胞形态和免疫组织化学染色特征（独特的细胞内颗粒模式）表明，该抗体可能特异性地与肥大细胞发生反应。我们采用组织化学和免疫组织化学相结合的方法，进一步对这种名为RCJ102的抗体进行表征。从21天龄胎鼠头部顶端制备含颅骨、软结缔组织和皮肤的冰冻切片，从成年大鼠制备肺、肌肉、脂肪组织和小肠的冰冻切片。一些切片用RCJ102进行间接免疫荧光标记；相应切片用甲苯胺蓝进行组织化学标记。除小肠外，在所有组织中，组织化学鉴定的肥大细胞与用RCJ102标记的细胞之间存在直接对应关系，在小肠中，组织化学可检测到的肥大细胞未被RCJ102标记。这些结果表明，RCJ102抗体将是进一步阐明结缔组织和肠道黏膜肥大细胞之间异质性的一种有价值的新试剂。
