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黄连解毒汤中黄连素、黄芩苷和栀子苷治疗缺血性脑卒中的作用：一种整合代谢组学方法的研究。

Treatment Effects of Ischemic Stroke by Berberine, Baicalin, and Jasminoidin from Huang-Lian-Jie-Du-Decoction (HLJDD) Explored by an Integrated Metabolomics Approach.

机构信息

Jiangsu Key Laboratory of Bioactive Natural Product Research and State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, 210009 Nanjing, China.

Center for Molecular Metabolism, Nanjing University of Science and Technology, 200 Xiao Ling Wei Street, 210094 Nanjing, China.

出版信息

Oxid Med Cell Longev. 2017;2017:9848594. doi: 10.1155/2017/9848594. Epub 2017 Aug 14.

DOI:10.1155/2017/9848594
PMID:28894512
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5574319/
Abstract

Berberine, baicalin, and jasminoidin were major active ingredients of Huang-Lian-Jie-Du-Decoction (HLJDD), a famous prescription of traditional Chinese medicine (TCM), which has been used for the treatment of ischemic stroke. The aim of the present study was to classify their roles in the treatment effects of ischemic stroke. A rat model of middle cerebral artery occlusion (MCAO) was constructed to mimic ischemic stroke and treatment effects of berberine, baicalin, and jasminoidin, and HLJDD was assessed by neurologic deficit scoring, infarct volume, histopathology, immunohistochemistry, biochemistry, quantitative real-time polymerase chain reaction (qRT-PCR), and Western blotting. In addition, the H NMR metabolomics approach was used to assess the metabolic profiles, which combined with correlation network analysis successfully revealed metabolic disorders in ischemic stroke concerning the treatment of the three principal compounds from HLJDD for the first time. The combined results suggested that berberine, baicalin, and jasminoidin are responsible for the effectiveness of HLJDD on the treatment of ischemic stroke by amelioration of abnormal metabolism and regulation of oxidative stress, neuron autophagy, and inflammatory response. This integrated metabolomics approach showed its potential in understanding the function of complex formulae and clarifying the role of its components in the overall treatment effects.

摘要

小檗碱、黄芩苷和茉莉苷元是黄连解毒汤（HLJDD）的主要活性成分，黄连解毒汤是一种著名的中药方剂，用于治疗缺血性中风。本研究旨在探讨其在缺血性中风治疗作用中的分类。构建大脑中动脉闭塞（MCAO）大鼠模型模拟缺血性中风，用神经功能缺损评分、梗死体积、组织病理学、免疫组织化学、生物化学、实时定量聚合酶链反应（qRT-PCR）和 Western blot 评估小檗碱、黄芩苷和茉莉苷元及 HLJDD 的治疗作用。此外，还采用 1H NMR 代谢组学方法评估代谢谱，结合相关网络分析首次成功揭示了 HLJDD 治疗缺血性中风时涉及的代谢紊乱。综合结果表明，小檗碱、黄芩苷和茉莉苷元通过改善异常代谢和调节氧化应激、神经元自噬和炎症反应，负责 HLJDD 治疗缺血性中风的有效性。这种综合代谢组学方法显示了其在理解复杂配方功能和阐明其成分在整体治疗效果中的作用的潜力。

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2
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J Ethnopharmacol. 2016 Apr 2;181:8-19. doi: 10.1016/j.jep.2016.01.028. Epub 2016 Jan 22.
3
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