Lindgren Gustaf, Wennerberg Johan, Ekblad Lars
Division of Oto-rhino-laryngology, Head and Neck Surgery Lund Sweden.
Department of Clinical Sciences Lund Skåne University Hospital, Lund University Lund Sweden.
Laryngoscope Investig Otolaryngol. 2017 May 20;2(4):194-198. doi: 10.1002/lio2.83. eCollection 2017 Aug.
The existence of circulating tumor cells has emerged as an important factor for prognosis and survival. The CellSearch method is the only circulating tumor cell detection method approved by the US Food and Drug Administration for clinical use. It relies on the detection of EpCAM (epithelial cell adhesion molecule) and is approved for colon cancer, breast cancer and prostate cancer. We now investigated whether CellSearch can be used to quantify circulating tumor cells in head and neck squamous cell cancer.
In this study, we investigated the expression of EpCAM in 12 head and neck squamous cell cancer cell lines using Western blot and how this affected their detectability with CellSearch in peripheral blood.
We found a great variation in the expression of EpCAM between our head and neck squamous cell cancer cell lines. This was accompanied by variations in counting efficiency.
We suggest that for reliable quantification of circulating tumor cells in blood from patients with head and neck squamous cell cancer cell, an epitope independent method is preferable.
NA.
循环肿瘤细胞的存在已成为影响预后和生存的一个重要因素。CellSearch方法是美国食品药品监督管理局批准的唯一可用于临床的循环肿瘤细胞检测方法。该方法依赖于上皮细胞粘附分子（EpCAM）检测，已被批准用于结肠癌、乳腺癌和前列腺癌。我们现研究CellSearch方法是否可用于对头颈部鳞状细胞癌患者的循环肿瘤细胞进行定量检测。
在本研究中，我们使用蛋白质免疫印迹法研究了12种头颈部鳞状细胞癌细胞系中EpCAM的表达情况，以及这如何影响其在外周血中被CellSearch方法检测到的能力。
我们发现头颈部鳞状细胞癌细胞系之间EpCAM的表达存在很大差异，这伴随着计数效率的变化。
我们建议，要对头颈部鳞状细胞癌患者血液中的循环肿瘤细胞进行可靠定量，采用一种不依赖表位的方法更为可取。
无。
