Huszar E, Herjavecz I, Debreczeni L A
Clinico-Pharmacological Unit, National Koranyi Institute for TBC and Pulmonology, Budapest, Hungary.
Bull Eur Physiopathol Respir. 1987;23 Suppl 10:115s-117s.
Numbers of beta-adrenoceptors in intact human lymphocytes of asthmatics treated continuously with the usual doses of beta-agonists have been estimated. Lymphocytes were isolated by density gradient centrifugation as described by BOYUM [4]. The binding experiment was performed with (-)3H-dihydroalprenolol which is a high affinity beta-adrenoceptor antagonist. There was no difference between the mean values of the binding sites of asthmatic patients and those of the normal volunteers. In asthmatic patients, a significant correlation was found between the density of receptors and the percentage increase of FEV1 after salbutamol inhalation. Symptoms of drug tolerance were not observed in patients treated with therapeutic doses of beta-agonists.
已对持续接受常规剂量β受体激动剂治疗的哮喘患者完整人淋巴细胞中的β肾上腺素能受体数量进行了评估。淋巴细胞通过如博伊姆[4]所述的密度梯度离心法分离。结合实验使用（-）3H-二氢心得舒进行，其为高亲和力β肾上腺素能受体拮抗剂。哮喘患者与正常志愿者的结合位点平均值之间没有差异。在哮喘患者中，发现受体密度与吸入沙丁胺醇后第一秒用力呼气容积（FEV1）的增加百分比之间存在显著相关性。接受治疗剂量β受体激动剂治疗的患者未观察到药物耐受性症状。
