Departments of Chemistry and Biology, Washington University in St. Louis, One Brookings Dr., St. Louis, MO, 63130, USA.
Donald Danforth Plant Science Center, 975 N Warson Rd, St. Louis, MO, 63132, USA.
Photosynth Res. 2017 Nov;134(2):183-192. doi: 10.1007/s11120-017-0428-1. Epub 2017 Sep 11.
Far-Red Light (FRL) acclimation is a process that has been observed in cyanobacteria and algae that can grow solely on light above 700 nm. The acclimation to FRL results in rearrangement and synthesis of new pigments and pigment-protein complexes. In this study, cyanobacteria containing chlorophyll f, Synechococcus sp. PCC 7335 and Halomicronema hongdechloris, were imaged as live cells with confocal microscopy. H. hongdechloris was further studied with hyperspectral confocal fluorescence microscopy (HCFM) and freeze-substituted thin-section transmission electron microscopy (TEM). Under FRL, phycocyanin-containing complexes and chlorophyll-containing complexes were determined to be physically separated and the synthesis of red-form phycobilisome and Chl f was increased. The timing of these responses was observed. The heterogeneity and eco-physiological response of the cells was noted. Additionally, a gliding motility for H. hongdechloris is reported.
远红光是一种在蓝细菌和藻类中观察到的现象，它们仅能在 700nm 以上的光线下生长。远红光适应会导致新的色素和色素蛋白复合物的重新排列和合成。在这项研究中，使用共聚焦显微镜对含有叶绿素 f 的蓝细菌、聚球藻 PCC 7335 和盐杆菌 H. hongdechloris 进行了活细胞成像。进一步对 H. hongdechloris 进行了超光谱共聚焦荧光显微镜（HCFM）和冷冻替代薄切片透射电子显微镜（TEM）研究。在远红光下，确定藻胆蛋白复合物和叶绿素复合物是物理分离的，并且红色形式的藻胆体和 Chl f 的合成增加。观察到这些反应的时间。注意到细胞的异质性和生态生理反应。此外，还报道了 H. hongdechloris 的滑行运动。
